Spinal cord injury presents a significant therapeutic challenge since the treatments available for this condition are largely vain. Recently the use of stem cells to treat this disease represents a promising new therapeutic strategy; indeed, a variety of stem cell treatments have been examined in animal models of CNS trauma. Cell treatment for neurodegenerative diseases was initially thought as a replacement strategy; however, increasing scientific evidences indicate rather a role in modulation of the inflammation that is responsible of as many damages as the primary disease itself. In this work a contusive mouse model of spinal cord injury (SCI) was used to test the therapeutic properties of mesenchymal stem cells derived from third trimester amniotic fluid (AFCs) previously characterized (1). Within the cultures used for transplantation only some were able to induce a significant motor recovery (2) respect to the vehicle-treated animals, and, surprising, all of these express the surface marker NG2. The motor functional recovery correlated with the preservation of the myelin in the ventral horn white matter of the spinal cord and with the increased levels of vascularization in the peri-lesion, and with an increase of the expression of VEGF and HIF-1α two days after cells transplantation respect to the PBS-treated animals indicates that an angiogenic pathway might be started by these cells possibly throughout the production of HGF.

NG2-positive cells from amniotic fluid ameliorate the functional recovery in a mouse model of spinal cord injury / G. Scesa, D. Cigognini, R. Adami, E. Nicora, A.M. Di Giulio, A. Gorio, D. Bottai. ((Intervento presentato al 15. convegno Congress of the Italian Society for Neuroscience tenutosi a Rome nel 2013.

NG2-positive cells from amniotic fluid ameliorate the functional recovery in a mouse model of spinal cord injury

G. Scesa;R. Adami;D. Bottai
2013

Abstract

Spinal cord injury presents a significant therapeutic challenge since the treatments available for this condition are largely vain. Recently the use of stem cells to treat this disease represents a promising new therapeutic strategy; indeed, a variety of stem cell treatments have been examined in animal models of CNS trauma. Cell treatment for neurodegenerative diseases was initially thought as a replacement strategy; however, increasing scientific evidences indicate rather a role in modulation of the inflammation that is responsible of as many damages as the primary disease itself. In this work a contusive mouse model of spinal cord injury (SCI) was used to test the therapeutic properties of mesenchymal stem cells derived from third trimester amniotic fluid (AFCs) previously characterized (1). Within the cultures used for transplantation only some were able to induce a significant motor recovery (2) respect to the vehicle-treated animals, and, surprising, all of these express the surface marker NG2. The motor functional recovery correlated with the preservation of the myelin in the ventral horn white matter of the spinal cord and with the increased levels of vascularization in the peri-lesion, and with an increase of the expression of VEGF and HIF-1α two days after cells transplantation respect to the PBS-treated animals indicates that an angiogenic pathway might be started by these cells possibly throughout the production of HGF.
5-ott-2013
NG2 ; Amniotic fluid stem cells ; spinal cord injury
Settore BIO/09 - Fisiologia
Settore BIO/14 - Farmacologia
Settore BIO/17 - Istologia
Settore BIO/11 - Biologia Molecolare
Italian Society for Neuroscience
NG2-positive cells from amniotic fluid ameliorate the functional recovery in a mouse model of spinal cord injury / G. Scesa, D. Cigognini, R. Adami, E. Nicora, A.M. Di Giulio, A. Gorio, D. Bottai. ((Intervento presentato al 15. convegno Congress of the Italian Society for Neuroscience tenutosi a Rome nel 2013.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/226194
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