Hypericum perforatum L. is a medicinal plant with antibacterial, antidepressant, anti-inflammatory, anti-cancer and antiviral and pharmacological properties. Its major metabolite, the prenylated acylphloroglucinol hyperforin, seems to be the main responsible for these activities. Many natural products show antimicrobial activity against bacteria, but only a small number of these are also inhibitors or disrupters of the biofilm. Biofilm is a mono-or multilayer of cells embedded within a matrix of extracellular polymeric material formation. Medical devices such as catheters, peacemakers and prosthetic heart valves can become colonized by microorganisms which form a biofilm, exposing patients at risk for local and systemic infectious complications. The aim of this study was to investigate the antibacterial activity of hyperforin and its hydrogenated derivative (octahydrohyperforin) against bacterial biofilms. The two compounds were tested for inhibition of bacterial growth and biofilm formation against a panel of clinically relevant pathogens comprising American Type Culture Collection (ATCC) strain: Staphylococcus aureus ATCC 29213, Staphylococcus aureus methicillin resistant ATCC 43300 (MRSA), Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922 and clinical isolate of MRSA, Staphylococcus aureus Ig5. Biofilm were developed in 96-well microtiter plates and the Minimum Biofilm Inhibitory Concentration (MBIC), which is the lowest concentration of an antimicrobial agent that results in no detectable biofilm growth, for synthesized compounds were determined using XTT reduction assay. The experimental details and results of the study are reported in this communication

In vitro activity of Hyperforin and Octahydroiperforin against planktonic and biofilm bacteria / B. Schiavone, F. Corbo, A. Rosato, C. Franchini, L. Verotta. ((Intervento presentato al 2. convegno International Symposium on Natural Products in Cancer Prevention and Therapy tenutosi a Napoli nel 2013.

In vitro activity of Hyperforin and Octahydroiperforin against planktonic and biofilm bacteria

L. Verotta
Ultimo
2013

Abstract

Hypericum perforatum L. is a medicinal plant with antibacterial, antidepressant, anti-inflammatory, anti-cancer and antiviral and pharmacological properties. Its major metabolite, the prenylated acylphloroglucinol hyperforin, seems to be the main responsible for these activities. Many natural products show antimicrobial activity against bacteria, but only a small number of these are also inhibitors or disrupters of the biofilm. Biofilm is a mono-or multilayer of cells embedded within a matrix of extracellular polymeric material formation. Medical devices such as catheters, peacemakers and prosthetic heart valves can become colonized by microorganisms which form a biofilm, exposing patients at risk for local and systemic infectious complications. The aim of this study was to investigate the antibacterial activity of hyperforin and its hydrogenated derivative (octahydrohyperforin) against bacterial biofilms. The two compounds were tested for inhibition of bacterial growth and biofilm formation against a panel of clinically relevant pathogens comprising American Type Culture Collection (ATCC) strain: Staphylococcus aureus ATCC 29213, Staphylococcus aureus methicillin resistant ATCC 43300 (MRSA), Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922 and clinical isolate of MRSA, Staphylococcus aureus Ig5. Biofilm were developed in 96-well microtiter plates and the Minimum Biofilm Inhibitory Concentration (MBIC), which is the lowest concentration of an antimicrobial agent that results in no detectable biofilm growth, for synthesized compounds were determined using XTT reduction assay. The experimental details and results of the study are reported in this communication
lug-2013
Settore CHIM/06 - Chimica Organica
Phytochemical Society of Europe
In vitro activity of Hyperforin and Octahydroiperforin against planktonic and biofilm bacteria / B. Schiavone, F. Corbo, A. Rosato, C. Franchini, L. Verotta. ((Intervento presentato al 2. convegno International Symposium on Natural Products in Cancer Prevention and Therapy tenutosi a Napoli nel 2013.
Conference Object
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/224282
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact