A purine nucleoside phosphorylase from Aeromonas hydrophila was cloned, expressed in Escherichia coll and purified. The enzyme showed a good stability and a broad substrate specificity towards modified purine ribonucleosides. The enzyme was used for the synthesis of purine nucleosides structurally related to compds. of biol. interest via a "one pot, one enzyme" transglycosylation reaction using 7-Me guanosine iodide as the sugar donor and 6-substituted purines as acceptor.
Sintesi chemoenzimatica di nucleosidi / C.F. Morelli, D. Ubiali, I. Serra, M. Biagiotti, G. Borghese, V.M. Pappalardo, A.M. Albertini, G. Speranza. - In: LA CHIMICA E L'INDUSTRIA. - ISSN 0009-4315. - 94:8(2012), pp. 108-111.
Sintesi chemoenzimatica di nucleosidi
C.F. MorelliPrimo
;I. Serra;M. Biagiotti;G. Borghese;V.M. Pappalardo;G. SperanzaUltimo
2012
Abstract
A purine nucleoside phosphorylase from Aeromonas hydrophila was cloned, expressed in Escherichia coll and purified. The enzyme showed a good stability and a broad substrate specificity towards modified purine ribonucleosides. The enzyme was used for the synthesis of purine nucleosides structurally related to compds. of biol. interest via a "one pot, one enzyme" transglycosylation reaction using 7-Me guanosine iodide as the sugar donor and 6-substituted purines as acceptor.
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