A promising approach for musculoskeletal repair and regeneration is mesenchymal-stem-cell- (MSC-)based tissue engineering. The aim of the study was to apply a simple protocol based on mincing the umbilical cord (UC), without removing any blood vessels or using any enzymatic digestion, to rapidly obtain an adequate number of multipotent UC-MSCs. We obtained, at passage 1 (P1), a mean value of cells (SD 0,4) from each UC. At immunophenotypic characterization, cells were positive for CD73, CD90, CD105, CD44, CD29, and HLA-I and negative for CD34 and HLA-class II, with a subpopulation negative for both HLA-I and HLA-II. Newborn origin and multilineage potential toward bone, fat, cartilage, and muscle was demonstrated. Telomere length was similar to that of bone-marrow (BM) MSCs from young donors. The results suggest that simply collecting UC-MSCs at P1 from minced umbilical cord fragments allows to achieve a valuable population of cells suitable for orthopaedic tissue engineering.

Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering: an in vitro study / A. Marmotti, S. Mattia, M. Bruzzone, S. Buttiglieri, A. Risso, D.E. Bonasia, D. Blonna, F. Castoldi, R. Rossi, C. Zanini, E. Ercole, E. Defabiani, C. Tarella, G.M. Peretti. - In: STEM CELLS INTERNATIONAL. - ISSN 1687-9678. - (2012), pp. 326813.1-326813.13.

Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering: an in vitro study

C. Tarella;G.M. Peretti
Ultimo
2012

Abstract

A promising approach for musculoskeletal repair and regeneration is mesenchymal-stem-cell- (MSC-)based tissue engineering. The aim of the study was to apply a simple protocol based on mincing the umbilical cord (UC), without removing any blood vessels or using any enzymatic digestion, to rapidly obtain an adequate number of multipotent UC-MSCs. We obtained, at passage 1 (P1), a mean value of cells (SD 0,4) from each UC. At immunophenotypic characterization, cells were positive for CD73, CD90, CD105, CD44, CD29, and HLA-I and negative for CD34 and HLA-class II, with a subpopulation negative for both HLA-I and HLA-II. Newborn origin and multilineage potential toward bone, fat, cartilage, and muscle was demonstrated. Telomere length was similar to that of bone-marrow (BM) MSCs from young donors. The results suggest that simply collecting UC-MSCs at P1 from minced umbilical cord fragments allows to achieve a valuable population of cells suitable for orthopaedic tissue engineering.
Settore MED/33 - Malattie Apparato Locomotore
2012
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/209469
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