The purpose of this study was twofold: first, to evaluate the myoblast labeling of various 99mTc complexes and to select the complex that best accomplishes this labeling, and second to evaluate the biodistribution of myoblasts labeled with this complex using mice with MDX muscular dystrophy (the murine homologue of Duchenne's muscular dystrophy). The following ligands were used to prepare the corresponding 99mTc complexes: hexakis-methoxy-isobutyl-isonitrile (MIBI), bis(2-ethoxyethyl)diphosphinoethane (Tf), (RR,SS)-4,8-diaza-3,6,6,9-tetramethyl-undecane-2,10-dione-bisoxime (HM-PAO), bis(N-ethyl)dithiocarbamate (NEt), and bis(N-ethoxy, N-ethyl)dithiocarbamate (NOEt). One million murine myoblasts were incubated for 30-60 minutes with 5 mCi of each of the 99mTc complexes prepared from the above ligands. Viability was assessed by microscopic counting after trypan blue staining, and the radioactivity absorbed in the cells was measured after centrifugation. The compound with the highest uptake in cellular pellets was [99mTc]N-NOEt. The biodistribution of myoblasts labeled with this complex was evaluated after intraaortic injection in dystrophic mice. Such an approach has the potential of effecting widespread gene transfer through the bloodstream to muscles lacking dystrophin.

Biodistribution studies of 99mTc-labeled myoblasts in a murine model of muscular dystrophy / F.R. Colombo, Y. Torrente, R. Casati, R. Benti, S.P. Corti, S. Salani, M.G. D'Angelo, A. DeLiso, G. Scarlato, N. Bresolin, P. Gerundini. - In: NUCLEAR MEDICINE AND BIOLOGY. - ISSN 0969-8051. - 28:8(2001 Nov), pp. 935-940. [10.1016/S0969-8051(01)00256-6]

Biodistribution studies of 99mTc-labeled myoblasts in a murine model of muscular dystrophy

Y. Torrente
Secondo
;
S.P. Corti;N. Bresolin
Penultimo
;
2001

Abstract

The purpose of this study was twofold: first, to evaluate the myoblast labeling of various 99mTc complexes and to select the complex that best accomplishes this labeling, and second to evaluate the biodistribution of myoblasts labeled with this complex using mice with MDX muscular dystrophy (the murine homologue of Duchenne's muscular dystrophy). The following ligands were used to prepare the corresponding 99mTc complexes: hexakis-methoxy-isobutyl-isonitrile (MIBI), bis(2-ethoxyethyl)diphosphinoethane (Tf), (RR,SS)-4,8-diaza-3,6,6,9-tetramethyl-undecane-2,10-dione-bisoxime (HM-PAO), bis(N-ethyl)dithiocarbamate (NEt), and bis(N-ethoxy, N-ethyl)dithiocarbamate (NOEt). One million murine myoblasts were incubated for 30-60 minutes with 5 mCi of each of the 99mTc complexes prepared from the above ligands. Viability was assessed by microscopic counting after trypan blue staining, and the radioactivity absorbed in the cells was measured after centrifugation. The compound with the highest uptake in cellular pellets was [99mTc]N-NOEt. The biodistribution of myoblasts labeled with this complex was evaluated after intraaortic injection in dystrophic mice. Such an approach has the potential of effecting widespread gene transfer through the bloodstream to muscles lacking dystrophin.
Animals ; Technetium ; Cells, Cultured ; Mice, Inbred C57BL ; Mice; Tissue Distribution ; Gene Therapy ; Cell Transplantation ; Muscular Dystrophy, Animal ; Muscle, Skeletal
Settore MED/26 - Neurologia
nov-2001
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/207216
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