A fully automated fluorometer which measures fluorescence depolarization is described. The instrument has been designed to work with standard microtiter sample holders normally used in clinical tests. The combination of a hybrid optical system, built with discrete optical elements and fiber bundles, and a digital Fourier analysis of the detected signals make the instrument quite sensitive and precise. The 96 samples are scanned in about 100 s, with a reproducibility of the measurement of the polarization ratio of about 10(-3) and a minimum content of fluorescein in the sample well of 20 pmol
A sensitive polaryzed fluorometer for clinical tests / P. Lago, L. Rovati, M. Corti. - In: IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT. - ISSN 0018-9456. - 43:4(1994), pp. 664-667.
A sensitive polaryzed fluorometer for clinical tests
M. CortiUltimo
1994
Abstract
A fully automated fluorometer which measures fluorescence depolarization is described. The instrument has been designed to work with standard microtiter sample holders normally used in clinical tests. The combination of a hybrid optical system, built with discrete optical elements and fiber bundles, and a digital Fourier analysis of the detected signals make the instrument quite sensitive and precise. The 96 samples are scanned in about 100 s, with a reproducibility of the measurement of the polarization ratio of about 10(-3) and a minimum content of fluorescein in the sample well of 20 pmol
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