Analysis of Candida albicans cells using antibodies directed against Gas1p/Ggp1p, Saccharomyces cerevisiae homolog of Phr1p, revealed that Phr1p is a glycoprotein of about 88 kDa whose accumulation increases with the rise of external pH, This polypeptide is present both in the yeast form and during germ tube induction, In the Phr1(-) cells at pH 8 the solubility of glucans in alkali is greatly affected. In the parental strain the alkali-soluble/-insoluble glucan ratio shows a 50% decrease at pH 8 with respect to pH 4.5, whereas in the null mutant it is unchanged, indicating the lack of a polymer cross-linker activity induced by the rise of pH, The mutant has a sixfold increase in chitin level and is hypersensitive to calcofluor. Consistently with a role of chitin in strengthening the cell wall, Phr1(-) cells are more sensitive to nikkomycin Z than the parental strain.

Defects in assembly of the extracellular matrix are responsible for altered morphogenesis of a Candida albicans phr1 mutant / L. Popolo, M. Vai. - In: JOURNAL OF BACTERIOLOGY. - ISSN 0021-9193. - 180:1(1998), pp. 163-166.

Defects in assembly of the extracellular matrix are responsible for altered morphogenesis of a Candida albicans phr1 mutant

L. Popolo
Primo
;
1998

Abstract

Analysis of Candida albicans cells using antibodies directed against Gas1p/Ggp1p, Saccharomyces cerevisiae homolog of Phr1p, revealed that Phr1p is a glycoprotein of about 88 kDa whose accumulation increases with the rise of external pH, This polypeptide is present both in the yeast form and during germ tube induction, In the Phr1(-) cells at pH 8 the solubility of glucans in alkali is greatly affected. In the parental strain the alkali-soluble/-insoluble glucan ratio shows a 50% decrease at pH 8 with respect to pH 4.5, whereas in the null mutant it is unchanged, indicating the lack of a polymer cross-linker activity induced by the rise of pH, The mutant has a sixfold increase in chitin level and is hypersensitive to calcofluor. Consistently with a role of chitin in strengthening the cell wall, Phr1(-) cells are more sensitive to nikkomycin Z than the parental strain.
English
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
1998
180
1
163
166
Pubblicato
Periodico con rilevanza internazionale
info:eu-repo/semantics/article
Defects in assembly of the extracellular matrix are responsible for altered morphogenesis of a Candida albicans phr1 mutant / L. Popolo, M. Vai. - In: JOURNAL OF BACTERIOLOGY. - ISSN 0021-9193. - 180:1(1998), pp. 163-166.
none
Prodotti della ricerca::01 - Articolo su periodico
2
262
Article (author)
no
L. Popolo, M. Vai
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/200633
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 36
social impact