The cell cycle modulated protein gp115 (115 kDa, isoelectric point about 4.8-5) of Saccharomyces cerevisiae undergoes various post-translational modifications. It is N-glycosylated during its maturation along the secretory pathway where an intermediary precursor of 100 kDa (p100), dynamically related to the mature gp115 protein, is detected at the level of endoplasmic reticulum. Moreover, we have shown by the use of metabolic labeling with [35S]methionine, [3H]palmitic acid and myo-[3H]inositol combined with high resolution two-dimensional gel electrophoresis and immunoprecipitation with a specific antiserum, that gp115 is one of the major palmitate- and inositol-containing proteins in yeast. These results, and the susceptibility of gp115 to phosphatidylinositol-specific phospholipase C treatment strongly indicate that gp115 contains the glycosylphosphatidylinositol (GPI) structure as membrane anchor domain. The two-dimensional analysis of the palmitate- and inositol-labeled proteins has also allowed the characterization of other polypeptides which possibly contain a GPI structure.

THE CELL-CYCLE MODULATED GLYCOPROTEIN GP115 IS ONE OF THE MAJOR YEAST PROTEINS CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL / M. VAI, L. POPOLO, R. GRANDORI, E. LACANA, L. ALBERGHINA. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - 1038:3(1990), pp. 277-285.

THE CELL-CYCLE MODULATED GLYCOPROTEIN GP115 IS ONE OF THE MAJOR YEAST PROTEINS CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL

L. POPOLO
Secondo
;
1990

Abstract

The cell cycle modulated protein gp115 (115 kDa, isoelectric point about 4.8-5) of Saccharomyces cerevisiae undergoes various post-translational modifications. It is N-glycosylated during its maturation along the secretory pathway where an intermediary precursor of 100 kDa (p100), dynamically related to the mature gp115 protein, is detected at the level of endoplasmic reticulum. Moreover, we have shown by the use of metabolic labeling with [35S]methionine, [3H]palmitic acid and myo-[3H]inositol combined with high resolution two-dimensional gel electrophoresis and immunoprecipitation with a specific antiserum, that gp115 is one of the major palmitate- and inositol-containing proteins in yeast. These results, and the susceptibility of gp115 to phosphatidylinositol-specific phospholipase C treatment strongly indicate that gp115 contains the glycosylphosphatidylinositol (GPI) structure as membrane anchor domain. The two-dimensional analysis of the palmitate- and inositol-labeled proteins has also allowed the characterization of other polypeptides which possibly contain a GPI structure.
(S. cerevisiae); Acylation; Glycoprotein; Glycosylphosphatidylinositol; Membrane anchor
Settore BIO/11 - Biologia Molecolare
1990
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/200568
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