The gp51-p30 glycoprotein constituting BLV envelope was expressed in Sf-21 insect cells by means of recombinant baculoviruses. Post-infection cell lysates mere analyzed, in order to define the immunologic reactivity of recombinant products. Oligosaccharide chains, containing N-acetylglucosamine, mannose, galactose and sialic acid mere found on recombinant gp51-p30. In order to investigate the timing of transcription and translation of the glycoprotein, kinetic assays were carried out on cell lysates and directly in situ on Sf-21 cells during the course of baculovirus infection, The use of different solubilizing reagents mas also evaluated in order to rescue recombinant glycoprotein from its subcellular location.

Expression of bovine leukemia virus ENV glycoprotein in insect cells by recombinant baculovirus / S. Russo, L. Montermini, T. Berkovitz-Siman, W. Ponti, G. Poli. - In: FEBS LETTERS. - ISSN 0014-5793. - 436:1(1998 Sep), pp. 11-16.

Expression of bovine leukemia virus ENV glycoprotein in insect cells by recombinant baculovirus

G. Poli
Ultimo
1998

Abstract

The gp51-p30 glycoprotein constituting BLV envelope was expressed in Sf-21 insect cells by means of recombinant baculoviruses. Post-infection cell lysates mere analyzed, in order to define the immunologic reactivity of recombinant products. Oligosaccharide chains, containing N-acetylglucosamine, mannose, galactose and sialic acid mere found on recombinant gp51-p30. In order to investigate the timing of transcription and translation of the glycoprotein, kinetic assays were carried out on cell lysates and directly in situ on Sf-21 cells during the course of baculovirus infection, The use of different solubilizing reagents mas also evaluated in order to rescue recombinant glycoprotein from its subcellular location.
baculovirus ; biotechnology ; bovine leukemia virus ; glycosylation ; recombinant glycoprotein
Settore VET/05 - Malattie Infettive degli Animali Domestici
set-1998
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/197857
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 8
  • ???jsp.display-item.citation.isi??? 7
social impact