Many cellular processes rely on the ordered assembly of macromolecular structures. Here, we uncover an unexpected link between two such processes, endocytosis and transcription. Many endocytic proteins, including eps15, epsin1, the clathrin assembly lymphoid myeloid leukemia (CALM), and alpha-adaptin, accumulate in the nucleus when nuclear export is inhibited. Endocytosis and nucleocytoplasmic shuttling of endocytic proteins are apparently independent processes, since inhibition of endocytosis did not appreciably alter nuclear translocation of endocytic proteins, and blockade of nuclear export did not change the initial rate of endocytosis. In the nucleus, eps15 and CALM acted as positive modulators of transcription in a GAL4-based transactivation assay, thus raising the intriguing possibility that some endocytic proteins play a direct or indirect role in transcriptional regulation.
Nucleocytoplasmic shuttling of endocytic proteins / M. Vecchi, S. Polo, V. Poupon, J.W. van de Loo, A. Benmerah, P.P. Di Fiore. - In: THE JOURNAL OF CELL BIOLOGY. - ISSN 0021-9525. - 153:7(2001 Jun 25), pp. 1511-1517. [10.1083/jcb.153.7.1511]
Nucleocytoplasmic shuttling of endocytic proteins
S. PoloSecondo
;P.P. Di Fiore
2001
Abstract
Many cellular processes rely on the ordered assembly of macromolecular structures. Here, we uncover an unexpected link between two such processes, endocytosis and transcription. Many endocytic proteins, including eps15, epsin1, the clathrin assembly lymphoid myeloid leukemia (CALM), and alpha-adaptin, accumulate in the nucleus when nuclear export is inhibited. Endocytosis and nucleocytoplasmic shuttling of endocytic proteins are apparently independent processes, since inhibition of endocytosis did not appreciably alter nuclear translocation of endocytic proteins, and blockade of nuclear export did not change the initial rate of endocytosis. In the nucleus, eps15 and CALM acted as positive modulators of transcription in a GAL4-based transactivation assay, thus raising the intriguing possibility that some endocytic proteins play a direct or indirect role in transcriptional regulation.File | Dimensione | Formato | |
---|---|---|---|
J Cell Biol-2001-Vecchi-1511-8.pdf
accesso aperto
Tipologia:
Publisher's version/PDF
Dimensione
314.97 kB
Formato
Adobe PDF
|
314.97 kB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.