1. Purinergic and adrenergic components of sympathetic neurotransmission and contractile responses to exogenous alpha,beta-methylene ATP and noradrenaline have been investigated in the seminal vesicles of 1-day (new-born), 2-weeks (young), 12-weeks (adult) and 2-years old (aged) guinea-pigs. 2. In seminal vesicles of new-born guinea-pigs electrical field stimulation (EFS; 80 V, 0.5 ms for 30 s, 2-32 Hz) evoked tonic frequency-related contractions. In 2-weeks old guinea-pigs the tonic contraction masked an initial phasic component of the neurogenic responses, whereas in 12-weeks and 2-years old guinea-pigs, neurogenic responses were biphasic, a phasic response being followed by a tonic contraction. In all experimental groups, prazosin (10(-6) M) blocked the tonic contraction while desensitization of P2X receptors by alpha,beta-methylene ATP (10(-4) M) abolished the phasic responses. 3. The phasic purinergic component of the neurogenic response was significantly higher in 12-weeks and 2-years old animals, compared with 2-weeks old guinea-pigs. At 32 Hz phasic contractions were (mN mg(-1) tissue): 0.047+/-0.012, 0.018+/-0.040 and 0.147+/-0.026 in 2-weeks, 12-weeks and 2-years old guinea-pigs, respectively. In contrast, the tonic adrenergic component of the neurogenic contraction significantly declined at 12-weeks and 2-years compared with 2-weeks old guinea-pigs. 4. Contractile responses (mN mg(-1) tissue) to the highest concentration of alpha,beta-methylene ATP tested were significantly higher in 2-weeks (0.248+/-0.022) than in 1-day old animals (0.113+/-0.012) and decreased in 12-weeks (0.163+/-0.016) and 2-years old guinea-pigs (0.200+/-0.008). The pD2 values for the purinoceptor agonist were also significantly lower in adult (4.74+/-0.20) and aged guinea-pigs (5.22+/-0.08) compared with 2-weeks old animals (5.91+/-0.27). Conversely, responses to the highest concentration of noradrenaline gradually decreased with age, without significant changes in the pD2 values. Contractile responses to KCl (240 mM) did not differ significantly between the experimental groups. 5. These results demonstrate age-related changes in purinergic and adrenergic components of sympathetic neurotransmission in the guinea-pig seminal vesicles. The purinergic component is absent in new-born animals and it appears fully developed in adult and old guinea-pigs, while the adrenergic component decreases with age. Pre- and postjunctional mechanisms contributing to the age-related changes of sympathetic neurotransmission are discussed.
Age-related changes in purinergic and adrenergic components of sympathetic neurotransmission in guinea-pig seminal vesicles / C. Pinna, A. Rubino, G. Burnstock. - In: BRITISH JOURNAL OF PHARMACOLOGY. - ISSN 0007-1188. - 122:7(1997), pp. 1411-1416.
|Titolo:||Age-related changes in purinergic and adrenergic components of sympathetic neurotransmission in guinea-pig seminal vesicles|
PINNA, CHRISTIAN (Primo)
|Parole Chiave:||Purinergic ; Adrenergic transmission ; seminal vesicles|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||1997|
|Appare nelle tipologie:|