Subcellular liver fractions from rats receiving a subcutaneous injection of turpentine, which causes a local inflammation, show an increased synthesis of Prostaglandin E2 and Prostaglandin F2 alpha which reaches a peak 90 minutes and 3 hours after treatment, respectively. Stimulation of phospholipase A2 activity of liver cell preparations seems to be responsible for the supply of arachidonic acid necessary to feed PG synthesis: this stimulation is accompanied by unchanged levels of diacylglycerol lipase, diacylglycerol kinase and protein kinase C activities and by an unchanged content of diacylglycerol in the liver tissue. This picture does not favour the hypothesis of an involvement of phospholipase C in the early stages after turpentine treatment. Determinations of GTP-ase activity in plasma membrane-rich liver preparations give ambiguous results, which do not allow any conclusion on the possible role of G-proteins in phospholipase A2 activation.

Rat liver eicosanoid synthesis during turpentine-induced inflammation / R. Piccoletti, D. Arienti, P. Bendinelli, A. Bernelli-Zazzera. - In: SECOND MESSENGER AND PHOSPHOPROTEINS. - ISSN 0895-7479. - 13:2-3(1991), pp. 141-55-155.

Rat liver eicosanoid synthesis during turpentine-induced inflammation

P. Bendinelli
Penultimo
;
1991

Abstract

Subcellular liver fractions from rats receiving a subcutaneous injection of turpentine, which causes a local inflammation, show an increased synthesis of Prostaglandin E2 and Prostaglandin F2 alpha which reaches a peak 90 minutes and 3 hours after treatment, respectively. Stimulation of phospholipase A2 activity of liver cell preparations seems to be responsible for the supply of arachidonic acid necessary to feed PG synthesis: this stimulation is accompanied by unchanged levels of diacylglycerol lipase, diacylglycerol kinase and protein kinase C activities and by an unchanged content of diacylglycerol in the liver tissue. This picture does not favour the hypothesis of an involvement of phospholipase C in the early stages after turpentine treatment. Determinations of GTP-ase activity in plasma membrane-rich liver preparations give ambiguous results, which do not allow any conclusion on the possible role of G-proteins in phospholipase A2 activation.
Animals; Microsomes, Liver; Turpentine; Subcellular Fractions; Phospholipases A2; Hepatitis, Animal; Diglycerides; Dinoprost; Guanosine Triphosphate; Rats; Rats, Inbred Strains; Dinoprostone; Protein Kinase C; Male; Phospholipases A
Settore MED/04 - Patologia Generale
1991
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/195405
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