BEHAVIOR OF SOME LYSOSOMAL-ENZYMES IN THE PLASMA OF INSULIN-DEPENDENT DIABETIC-PATIENTS DURING ARTIFICIAL PANCREAS TREATMENT

The plasma levels of three lysosomal enzymes, β-D-N-acetylglucosaminidase, β-D-glucuronidase, and α-L-fucosidase, were fluorimetrically determined in seven insulin-dependent diabetic patients one day before, one day after, and during a two-day treatment with the artificial pancreas, at 4 to 5 h intervals. A statistically significant decrease of the plasma level of each enzymes was observed during artificial pancreas treatment. The extent of decrease was 30 to 35% for β-N-acetylglucosaminidase, 35 to 40% for β-D-glucuronidase, and 20 to 25% for α-L-fucosidase. The decrease occurred earlier (at the first day of treatment) for β-D-N-acetylglucosaminidase, and later (at the second day of treatment, and lasting to the first day after treatment) for the other two enzymes. These results suggest a direct connection between the lysosomal apparatus and insulin-controlled metabolic pathways, and a potential role for lysosomal enzymes as indicators of the metabolic compensation in diabetes.