The immunopurified yeast DNA polymerase--DNA primase complex is constituted by DNA polymerase I polypeptides and by three other protein species, called p74, p58 and p48, which we show to be immunologically unrelated. The gene encoding the p48 polypeptide has been identified by immunological screening of a lambda gt11 yeast genomic DNA library. Antiserum specific for p48 inhibits DNA primase, and immunoreactive, inhibitory antibodies are affinity-purified by the clone-encoded protein, thus relating the p48 polypeptide to DNA primase activity. The entire gene has been cloned, and the 1.45-kb p48 mRNA is overproduced in cells containing the gene in high copy number. Gene disruption and Southern hybridization experiments demonstrate that the p48 protein is encoded by a single gene and it performs an essential function.

Yeast DNA polymerase--DNA primase complex; cloning of PRI 1, a single essential gene related to DNA primase activity / G. Lucchini, S. Francesconi, M. Foiani, G. Badaracco, P. Plevani. - In: EMBO JOURNAL. - ISSN 0261-4189. - 6:3(1987 Mar), pp. 737-42-742.

Yeast DNA polymerase--DNA primase complex; cloning of PRI 1, a single essential gene related to DNA primase activity

M. Foiani;P. Plevani
Ultimo
1987

Abstract

The immunopurified yeast DNA polymerase--DNA primase complex is constituted by DNA polymerase I polypeptides and by three other protein species, called p74, p58 and p48, which we show to be immunologically unrelated. The gene encoding the p48 polypeptide has been identified by immunological screening of a lambda gt11 yeast genomic DNA library. Antiserum specific for p48 inhibits DNA primase, and immunoreactive, inhibitory antibodies are affinity-purified by the clone-encoded protein, thus relating the p48 polypeptide to DNA primase activity. The entire gene has been cloned, and the 1.45-kb p48 mRNA is overproduced in cells containing the gene in high copy number. Gene disruption and Southern hybridization experiments demonstrate that the p48 protein is encoded by a single gene and it performs an essential function.
DNA Primase; Protein Biosynthesis; DNA Restriction Enzymes; Genes; Genes, Fungal; Escherichia coli; Transcription, Genetic; RNA Nucleotidyltransferases; Nucleic Acid Hybridization; Saccharomyces cerevisiae; Cloning, Molecular
Settore BIO/11 - Biologia Molecolare
mar-1987
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/189027
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