Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.
An improved procedure for in situ RTPCR / L. Volpi, L. Doneda, M. G. Grimoldi, E. Pollina, L. Larizza. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - 10:1(1996 Feb), pp. 63-66.
An improved procedure for in situ RTPCR
L. VolpiPrimo
;L. DonedaSecondo
;M. G. Grimoldi;L. LarizzaUltimo
1996
Abstract
Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.
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