Flow cytometric analysis of DNA content was performed on 72 formalin-fixed, paraffin wax-embedded samples of canine mammary tumours. DNA content was correlated with histological appearance, stage of the tumour, and survival after mastectomy. All benign tumours (14 cases) were diploid. Amongst the malignant tumours, a higher incidence of aneuploid tumours (50 per cent) was observed in cases with lymph nodal metastases in comparison with those without metastases (26 per cent). All dogs with benign tumours survived for at least two years after mastectomy and so did 26 (81 per cent) of 32 DNA diploid malignant cases without metastases. A 67 per cent (eight of 12 cases) survival rate was found in the dogs with DNA aneuploid malignant tumours without metastases, while a very low survival rate (17 per cent) was observed in the group with malignant tumours with metastases, independently of the extent of ploidy.

Flow cytometric analysis of cellular DNA content in paraffin wax-embedded specimens of canine mammary tumours / E. Scanziani, M. Caniatti, S. Sen, E. Erba, F. Cairoli, M. Battocchio. - In: JOURNAL OF COMPARATIVE PATHOLOGY. - ISSN 0021-9975. - 105:1(1991 Jul), pp. 75-82-82. [10.1016/S0021-9975(08)80062-X]

Flow cytometric analysis of cellular DNA content in paraffin wax-embedded specimens of canine mammary tumours

E. Scanziani
Primo
;
M. Caniatti
Secondo
;
F. Cairoli
Penultimo
;
M. Battocchio
Ultimo
1991

Abstract

Flow cytometric analysis of DNA content was performed on 72 formalin-fixed, paraffin wax-embedded samples of canine mammary tumours. DNA content was correlated with histological appearance, stage of the tumour, and survival after mastectomy. All benign tumours (14 cases) were diploid. Amongst the malignant tumours, a higher incidence of aneuploid tumours (50 per cent) was observed in cases with lymph nodal metastases in comparison with those without metastases (26 per cent). All dogs with benign tumours survived for at least two years after mastectomy and so did 26 (81 per cent) of 32 DNA diploid malignant cases without metastases. A 67 per cent (eight of 12 cases) survival rate was found in the dogs with DNA aneuploid malignant tumours without metastases, while a very low survival rate (17 per cent) was observed in the group with malignant tumours with metastases, independently of the extent of ploidy.
Animals; Lymphatic Metastasis; Dogs; S Phase; Mastectomy; Mammary Neoplasms, Experimental; Flow Cytometry; Ploidies; Female; DNA, Neoplasm
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/187456
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