EtOH [64-17-5] was estd. in human blood by the detn. of the difference in pH between blood samples without and with alc. dehydrogenase + aldehyde dehydrogenase. Relative std. deviation was <1.54% in the range of 10-40 mmol EtOH/L. Results were comparable with those obtained by head-space gas chromatog. The method is specific, rapid, and sensitive (∼20 mmol/L). MeOH, ethylene glycol, and iso-PrOH did not interfere with the detn.; propanol, butanol, and AcH did interfere with the detn.
The determination of ethanol in whole blood by differential pH measurements / E. Rovida, M. Luzzana, M. Ripamonti, A. Mosca, V. Gambaro, E. Marozzi, F. Lodi, L. Rossi-Bernardi, L.. - In: SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION. - ISSN 0036-5513. - 44:7(1984), pp. 611-615.
The determination of ethanol in whole blood by differential pH measurements
A. Mosca;V. Gambaro;E. Marozzi;F. Lodi;
1984
Abstract
EtOH [64-17-5] was estd. in human blood by the detn. of the difference in pH between blood samples without and with alc. dehydrogenase + aldehyde dehydrogenase. Relative std. deviation was <1.54% in the range of 10-40 mmol EtOH/L. Results were comparable with those obtained by head-space gas chromatog. The method is specific, rapid, and sensitive (∼20 mmol/L). MeOH, ethylene glycol, and iso-PrOH did not interfere with the detn.; propanol, butanol, and AcH did interfere with the detn.
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