During the conservation of feline semen, the freeze-thaw procedure in particular is responsible for inducing severe spermatozoal damage, which diminishes fertilizing ability. Therefore, cold-induced damage represents a limiting factor for the conservation of semen, particularly semen from felids, which are often affected by teratospermia. In this article, feline sperm characteristics are reported, with special reference to motility and morphology, which are more likely to be affected by conservation protocols; and moreover, the causes of cold-induced damages are described. Attention has been focused on methods to evaluate functional integrity of spermatozoa, and those applied to cat semen are reviewed. Among these, a rather recently developed technique involves fluorescent staining methods, and in particular chlortetracycline. The chlortetracycline assay applied to cryopreserved cat epididymal sperm shows that it is suitable to evaluate the functional status of cat sperm.
|Titolo:||Conservation of feline semen. Part 2. : Cold-induced damages on spermatozoal fertilizing ability|
|Autori interni:||LUVONI, GAIA CECILIA (Primo)|
|Parole Chiave:||Cat ; spermatozoa ; conservation ; cell damages|
|Settore Scientifico Disciplinare:||Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria|
|Data di pubblicazione:||2003|
|Digital Object Identifier (DOI):||10.1016/S1098-612X(03)00030-5|
|Appare nelle tipologie:||01 - Articolo su periodico|