We have investigated the relevance of actin rearrangement to neurotransmitter release, in neuroblastoma cell line SH-5Y5Y stimulated with carbachol (1 mM). Carbachol reversibly polymerizes actin and releases norepinephrine in undifferentiated SH-5Y5Y cells as well as in tetradecanoylphorbol 13-acetate (16 nM) and retinoic acid (5 μM) differentiated SH-5Y5Y cells. Microscopic analysis of F-actin distribution indicates polymerization of cortical actin. Prior treatment with iota toxin E from Clostridium perfringens inhibits both carbachol-induced actin polymerization and norepinephrine release, slightly affecting the basal actin network. These data suggest that actin polymerization is associated with norepinephrine release.
Is actin polymerization relevant to neurosecretion? A study on neuroblastoma cells / B. Viviani, C.L. Galli, M. Marinovich. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 223:3(1996), pp. 712-717.
Is actin polymerization relevant to neurosecretion? A study on neuroblastoma cells
B. VivianiPrimo
;C.L. GalliSecondo
;M. MarinovichUltimo
1996
Abstract
We have investigated the relevance of actin rearrangement to neurotransmitter release, in neuroblastoma cell line SH-5Y5Y stimulated with carbachol (1 mM). Carbachol reversibly polymerizes actin and releases norepinephrine in undifferentiated SH-5Y5Y cells as well as in tetradecanoylphorbol 13-acetate (16 nM) and retinoic acid (5 μM) differentiated SH-5Y5Y cells. Microscopic analysis of F-actin distribution indicates polymerization of cortical actin. Prior treatment with iota toxin E from Clostridium perfringens inhibits both carbachol-induced actin polymerization and norepinephrine release, slightly affecting the basal actin network. These data suggest that actin polymerization is associated with norepinephrine release.
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