IN VITRO INFLUENCE OF TWO CONCENTRATIONS OF KETONE BODIES ON DIFFERENT STEPS OF PHAGOCYTOSIS OF OVINE NEUTROPHILS Sartorelli P., Paltrinieri S., Agnes F. Istituto di Patologia Generale Veterinaria, Milano In previous works (1, 2) we have found that sheep neutrophil functions were affected differently when high concentrations of acetoacetate (4.8 mmol/l), -OH-butyrate (4.8 mmol/l), or acetoacetate (4.8 mmol/l) and -OH-butyrate (4.8 mmol/l) together were present. Because acetoacetate is a lithium salt, we also verified the effects of LiCl (4.8 mmol/l): in particular chemotaxis was depressed when both the ketone bodies were added simultaneously, while the uptake of latex particles was depressed only with -OH-butyrate, maybe due to the activating effect of LiCl. To verify the efficency of non-specific immunity in subketotic conditions, when infectious diseases are frequently detectable, we repeated these tests in the presence of lower concentrations of the ketone bodies (2.4 mmol/l). Furthermore, to complete the evaluation of the phagocytic process we studied the adherence and the respiratory burst of ovine neutrophils either at 4.8 mmol/l or at 2.4 mmol/l of the ketone bodies. Neutrophils were isolated from the blood of 8 sheep by centrifugation followed by two hypotonic lysis of erythrocytes; chemotaxis was measured in a modified Boyden chamber using the leading front method; phagocytosis was measured using polystyrene latex particles, with spectrophotometric evaluation after dioxan solubilization; adherence was measured in microtiter plate, along with the respiratory burst by means of superoxide production evaluation. Adherence and superoxide production were not affected by the ketone bodies or LiCl at the different concentrations employed. Even if a trend to depression of chemotaxis was detectable when the two ketone bodies were added simultaneously, no significative differences were found, due to strong individual variations. No variation in latex partcicle uptake ability was detectable using 2.4 mmol/l ketone bodies. In conclusion, while with high concentrations of ketone bodies some steps of the phagocytic process were undoubtably affected, the influence of lower concentration of ketone bodies, similar to those detectable in subketotic conditions, is much more variable among animals. This could suggest that non specific immunity is less involved in the immunodepressive status that characterize the subketotic condition, than other factors, such as specific immunity depression. (1) Paltrinieri S. et al., (1995), European Journal of Veterinary Pathology, 2 (Suppl): 48 (2) Paltrinieri S. et al., (1996), European Journal of Haematology, 57 (Suppl. 59):18

In vitro influence of two concentrations of ketone bodies on different steps of phagocytosis of ovine neutrophils / P. Sartorelli, S. Paltrinieri, F. Agnes. ((Intervento presentato al 15. convegno Atti 15° E.S.V.P. Congress tenutosi a Alghero nel 1997.

In vitro influence of two concentrations of ketone bodies on different steps of phagocytosis of ovine neutrophils

P. Sartorelli
Primo
;
S. Paltrinieri
Secondo
;
F. Agnes
Ultimo
1997

Abstract

IN VITRO INFLUENCE OF TWO CONCENTRATIONS OF KETONE BODIES ON DIFFERENT STEPS OF PHAGOCYTOSIS OF OVINE NEUTROPHILS Sartorelli P., Paltrinieri S., Agnes F. Istituto di Patologia Generale Veterinaria, Milano In previous works (1, 2) we have found that sheep neutrophil functions were affected differently when high concentrations of acetoacetate (4.8 mmol/l), -OH-butyrate (4.8 mmol/l), or acetoacetate (4.8 mmol/l) and -OH-butyrate (4.8 mmol/l) together were present. Because acetoacetate is a lithium salt, we also verified the effects of LiCl (4.8 mmol/l): in particular chemotaxis was depressed when both the ketone bodies were added simultaneously, while the uptake of latex particles was depressed only with -OH-butyrate, maybe due to the activating effect of LiCl. To verify the efficency of non-specific immunity in subketotic conditions, when infectious diseases are frequently detectable, we repeated these tests in the presence of lower concentrations of the ketone bodies (2.4 mmol/l). Furthermore, to complete the evaluation of the phagocytic process we studied the adherence and the respiratory burst of ovine neutrophils either at 4.8 mmol/l or at 2.4 mmol/l of the ketone bodies. Neutrophils were isolated from the blood of 8 sheep by centrifugation followed by two hypotonic lysis of erythrocytes; chemotaxis was measured in a modified Boyden chamber using the leading front method; phagocytosis was measured using polystyrene latex particles, with spectrophotometric evaluation after dioxan solubilization; adherence was measured in microtiter plate, along with the respiratory burst by means of superoxide production evaluation. Adherence and superoxide production were not affected by the ketone bodies or LiCl at the different concentrations employed. Even if a trend to depression of chemotaxis was detectable when the two ketone bodies were added simultaneously, no significative differences were found, due to strong individual variations. No variation in latex partcicle uptake ability was detectable using 2.4 mmol/l ketone bodies. In conclusion, while with high concentrations of ketone bodies some steps of the phagocytic process were undoubtably affected, the influence of lower concentration of ketone bodies, similar to those detectable in subketotic conditions, is much more variable among animals. This could suggest that non specific immunity is less involved in the immunodepressive status that characterize the subketotic condition, than other factors, such as specific immunity depression. (1) Paltrinieri S. et al., (1995), European Journal of Veterinary Pathology, 2 (Suppl): 48 (2) Paltrinieri S. et al., (1996), European Journal of Haematology, 57 (Suppl. 59):18
1997
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
European Society of Veterinary Pathology
In vitro influence of two concentrations of ketone bodies on different steps of phagocytosis of ovine neutrophils / P. Sartorelli, S. Paltrinieri, F. Agnes. ((Intervento presentato al 15. convegno Atti 15° E.S.V.P. Congress tenutosi a Alghero nel 1997.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/180681
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