Two gangliosides, representing 85% of total lipid-bound sialic acid, have been isolated from bovine buttermilk and characterized. Both contained long-chain base, glucose, galactose and sialic acid in the molar ratio 1:1:1:2, and gave, upon sialidase treatment, a neutral glycolipid, characterized as lactosylceramide. Partial acid hydrolysis, permethylation analysis and chromium trioxide oxidation indicated their basic oligosaccharide portion to be NeuAcα2 → 8NeuAcα2 → 3Galβ1 → 4Glc. The difference bteween the two forms was exclusively in the ceramide moiety of the molecule, one containing mainly long-chain (C22-C25) fatty acids and an equimolar proportion of C16 and C18 long-chain bases, and the other mainly palmitic acid and C18 long-chain base.
Characterization of two molecular species GD3 ganglioside from bovine buttermilk / B. Hauttecoeur, S. Sonnino, R. Ghidoni. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - 833:2(1985), pp. 303-307.
Characterization of two molecular species GD3 ganglioside from bovine buttermilk
S. SonninoSecondo
;R. GhidoniUltimo
1985
Abstract
Two gangliosides, representing 85% of total lipid-bound sialic acid, have been isolated from bovine buttermilk and characterized. Both contained long-chain base, glucose, galactose and sialic acid in the molar ratio 1:1:1:2, and gave, upon sialidase treatment, a neutral glycolipid, characterized as lactosylceramide. Partial acid hydrolysis, permethylation analysis and chromium trioxide oxidation indicated their basic oligosaccharide portion to be NeuAcα2 → 8NeuAcα2 → 3Galβ1 → 4Glc. The difference bteween the two forms was exclusively in the ceramide moiety of the molecule, one containing mainly long-chain (C22-C25) fatty acids and an equimolar proportion of C16 and C18 long-chain bases, and the other mainly palmitic acid and C18 long-chain base.
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