A new spectrophotometric cuvette holder to be used for subzero temperature is described. The device is easily adaptable to a commercial spectrophotometer and it was checked down to --40 degrees C. Satisfactory mixing of the reactants contained in the cuvette at low temperatures is attained using a special stirrer and suitable solution volumes. The rate of carbonmonoxyhemoglobin oxidation by K3Fe(CN)6 at different subzero temperatures has been studied using this apparatus; the results are in agreement with the extrapolated data at room temperature.

A new spectrophotometric cuvette holder for low temperature studies; its application to the study of carbonmonoxyhemoglobin oxidation rate / A. Mosca, A. Musetti, M. Samaja. - In: JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS. - ISSN 0165-022X. - 1:6(1979 Dec), pp. 319-326. [10.1016/0165-022X(79)90022-8]

A new spectrophotometric cuvette holder for low temperature studies; its application to the study of carbonmonoxyhemoglobin oxidation rate

A. Mosca
Primo
;
M. Samaja
Ultimo
1979

Abstract

A new spectrophotometric cuvette holder to be used for subzero temperature is described. The device is easily adaptable to a commercial spectrophotometer and it was checked down to --40 degrees C. Satisfactory mixing of the reactants contained in the cuvette at low temperatures is attained using a special stirrer and suitable solution volumes. The rate of carbonmonoxyhemoglobin oxidation by K3Fe(CN)6 at different subzero temperatures has been studied using this apparatus; the results are in agreement with the extrapolated data at room temperature.
antifreezing agents; carbonmonoxyhemoglobin oxidation; intermediate compounds; subzero spectrophotometry
Settore BIO/10 - Biochimica
dic-1979
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/175975
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