The N-type voltage-operated calcium channel has been characterized over the years as a high-threshold channel, with variable inactivation kinetics, and a unique ability to bind with high affinity and specificity omega-conotoxin GVIA and related toxins. This channel is particularly expressed in some neurons and endocrine cells, where it participates in several calcium-dependent processes, including secretion. Omega-conotoxin GVIA was instrumental not only for the biophysical and pharmacological characterization of N-type channels but also for the development of in vitro assays for studying N-type VOCC subcellular localization, biosynthesis, turnover, as well as short-and long-term regulation of its expression. We here summarize our studies on N-type VOCC expression in neurosecretory cells, with a major emphasis on recent data demonstrating the presence of N-type channels in intracellular secretory organelles and their recruitment to the cell surface during regulated exocytosis.
Metabolism and trafficking of N-type voltage-operated calcium channels in neurosecretory cells / E. Sher, P. Rosa, M. Francolini, A. Codignola, E. Morlacchi, E. Taverna, F. Giovannini, A. Brioschi, F. Clementi, M. W. McEnery, M. Passafaro. - In: JOURNAL OF BIOENERGETICS AND BIOMEMBRANES. - ISSN 0145-479X. - 30:4(1998 Aug), pp. 399-407.
|Titolo:||Metabolism and trafficking of N-type voltage-operated calcium channels in neurosecretory cells|
|Parole Chiave:||Animals ; Calcium ; Humans ; Biological Transport ; Calcium Channel Blockers ; Nerve Tissue Proteins ; Calcium Channels, N-Type ; Rats ; Calcium Channels ; Down-Regulation ; omega-Conotoxin GVIA ; Cell Membrane ; Membrane Potentials ; Peptides ; Up-Regulation ; Neurosecretory Systems ; Calcium Signaling ; PC12 Cells|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||ago-1998|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1023/A:1021945907635|
|Appare nelle tipologie:||01 - Articolo su periodico|