We report the evidence for the cellular uptake of gold nanoparticles via the phagocytosis mechanism in murine macrophage cells strongly supported by TEM and optical microscopy. Nanoparticles were prepared using several biocompatible molecules of choice (5-aminovaleric acid, l-DOPA, melatonin, and serotonin hydrochloride) as stabilizers for gold colloids. Their surface chemistry was fully characterized by UV−vis, ATR-FTIR, 1H NMR, and HR-MAS 1H NMR spectroscopies, and size distribution was determined by CPS disc centrifuge and TEM. Differences in coatings were evaluated against cellular uptake, and a preferential movement of macrophages toward 5-aminovaleric acid-modified gold nanoparticles was shown, leading to the fast accumulation of nanoparticles in the cytosol.
Phagocytosis of biocompatible gold nanoparticles / Z. Krpetic, F. Porta, E. Caneva, V. Dal Santo, G. Scari'. - In: LANGMUIR. - ISSN 0743-7463. - 26:18(2010), pp. 14799-14805. [10.1021/la102758f]
Phagocytosis of biocompatible gold nanoparticles
Z. Krpetic;F. Porta;E. Caneva;G. Scari'
2010
Abstract
We report the evidence for the cellular uptake of gold nanoparticles via the phagocytosis mechanism in murine macrophage cells strongly supported by TEM and optical microscopy. Nanoparticles were prepared using several biocompatible molecules of choice (5-aminovaleric acid, l-DOPA, melatonin, and serotonin hydrochloride) as stabilizers for gold colloids. Their surface chemistry was fully characterized by UV−vis, ATR-FTIR, 1H NMR, and HR-MAS 1H NMR spectroscopies, and size distribution was determined by CPS disc centrifuge and TEM. Differences in coatings were evaluated against cellular uptake, and a preferential movement of macrophages toward 5-aminovaleric acid-modified gold nanoparticles was shown, leading to the fast accumulation of nanoparticles in the cytosol.
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