The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.

Cytogenetic characterization and cell cycle analysis of three human colon adenocarcinoma cell lines: comparison between two- and three-dimensional cell culture systems / L.R. Roncoroni, L. Elli, M.T. Bardella, E. Dogliotti, M.G. Grimoldi, S. Viaggi, E. Erba, A. Zunino, L. Doneda. - In: CANCER INVESTIGATION. - ISSN 0735-7907. - 28:1(2010), pp. 7-12. [10.3109/07357900802653431]

Cytogenetic characterization and cell cycle analysis of three human colon adenocarcinoma cell lines: comparison between two- and three-dimensional cell culture systems

L.R. Roncoroni
Primo
;
L. Elli
Secondo
;
M.T. Bardella;E. Dogliotti;M.G. Grimoldi;L. Doneda
Ultimo
2010

Abstract

The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.
Cell cycle; CGH; Colon carcinoma; Cytogenetic analysis; Spheroids
Settore MED/08 - Anatomia Patologica
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/150615
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