The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.
Cytogenetic characterization and cell cycle analysis of three human colon adenocarcinoma cell lines : comparison between two- and three-dimensional cell culture systems / L.R. Roncoroni, L. Elli, M.T. Bardella, E. Dogliotti, M.G. Grimoldi, S. Viaggi, E. Erba, A. Zunino, L. Doneda. - In: CANCER INVESTIGATION. - ISSN 0735-7907. - 28:1(2010 Jan), pp. 7-12.
|Titolo:||Cytogenetic characterization and cell cycle analysis of three human colon adenocarcinoma cell lines : comparison between two- and three-dimensional cell culture systems|
RONCORONI, LEDA ROSALBA (Primo)
ELLI, LUCA (Secondo)
DONEDA, LUIGIA (Ultimo)
|Parole Chiave:||Cell cycle; CGH; Colon carcinoma; Cytogenetic analysis; Spheroids|
|Settore Scientifico Disciplinare:||Settore MED/08 - Anatomia Patologica|
|Data di pubblicazione:||gen-2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3109/07357900802653431|
|Appare nelle tipologie:||01 - Articolo su periodico|