The clinical success of the implants is related to their early osteo-integration which depends primarily on the properties of the surface. It is known that an increased implant-surface roughness significantly influences the osteoblastic response-, improving their adhesion and proliferation; same results have been described with bone marrow mesenchymal stem cells. Meanwhile, new techniques of surface treatment and coating deposition have been developed to prevent corrosion and debris formation which may causetissue inflammation, osteolysis and finally the loosening of the implant. In this study either standard hydroxyapatite (HA), or different kind of chemically modified titanium (TIT, TAA ) and silicon carbide obtained by plasma enhanced chemical vapor deposition (SiC-PECVD), a novel and promising biocompatible material for the covering of orthopaedic devices, were analyzed for their properties in supporting cell adhesion, proliferation and osteo-differentiation. Human adipose-derived stem cells (hASCs) were used for all these analyses since they possess a multi-differentiative potential and a reduced immunogenicity,features that make these cells useful and suitable model for these tests. We have assessed their ability to let hASC cells grow, both in non inductive and in osteo-inductive conditions, in comparison with the one cultured adhered on plastic (PA). Both undifferentiated and osteo-differentiated hASCs well colonized the tested biomaterials without showing any cytotoxic effect. HA, TIT, TAA and SiC-PECVD clearly possessed osteoinductive properties: indeed hASCs cultured on these biomaterials showed a significant (marked) increase of osteogenic markers such asalkaline phosphatase activity and calcified matrix deposition in comparison to cells cultured on plastic. This study suggest the use of hASC cells to test and to predict in vitro the compatibility and the osteo-inductive properties of biomaterials involved in orthopaedic application.
In vitro testing of biomaterials osteo-inductive properties by human adipose-derived stem cells / S. Lopa, L. De Girolamo, E. Arrigoni, D. Stanco, R. Chiesa, A.T. Brini. ((Intervento presentato al 95. convegno Congresso Nazionale della Società Italiana di Ortopedia e Traumatologia (SIOT) tenutosi a Roma nel 2010.
In vitro testing of biomaterials osteo-inductive properties by human adipose-derived stem cells
L. De GirolamoSecondo
;E. Arrigoni;D. Stanco;A.T. BriniUltimo
2010
Abstract
The clinical success of the implants is related to their early osteo-integration which depends primarily on the properties of the surface. It is known that an increased implant-surface roughness significantly influences the osteoblastic response-, improving their adhesion and proliferation; same results have been described with bone marrow mesenchymal stem cells. Meanwhile, new techniques of surface treatment and coating deposition have been developed to prevent corrosion and debris formation which may causetissue inflammation, osteolysis and finally the loosening of the implant. In this study either standard hydroxyapatite (HA), or different kind of chemically modified titanium (TIT, TAA ) and silicon carbide obtained by plasma enhanced chemical vapor deposition (SiC-PECVD), a novel and promising biocompatible material for the covering of orthopaedic devices, were analyzed for their properties in supporting cell adhesion, proliferation and osteo-differentiation. Human adipose-derived stem cells (hASCs) were used for all these analyses since they possess a multi-differentiative potential and a reduced immunogenicity,features that make these cells useful and suitable model for these tests. We have assessed their ability to let hASC cells grow, both in non inductive and in osteo-inductive conditions, in comparison with the one cultured adhered on plastic (PA). Both undifferentiated and osteo-differentiated hASCs well colonized the tested biomaterials without showing any cytotoxic effect. HA, TIT, TAA and SiC-PECVD clearly possessed osteoinductive properties: indeed hASCs cultured on these biomaterials showed a significant (marked) increase of osteogenic markers such asalkaline phosphatase activity and calcified matrix deposition in comparison to cells cultured on plastic. This study suggest the use of hASC cells to test and to predict in vitro the compatibility and the osteo-inductive properties of biomaterials involved in orthopaedic application.
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