Soft X-ray Contact Microscopy (SXCM) of Caenorhabditis elegans nematodes with typical length ∼800 μm and diameter ∼30 μm has been performed using the PALS laser source of wavelength λ = 1.314 μm and pulse duration τ(FWHM) = 400 ps. Pulsed soft X-rays were generated using molybdenum and gold targets with laser intensities I ≥ 1014 W/cm2. Images have been recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Cuticle features and several internal organs have been identified in the SXCM images including lateral field, cuticle annuli, pharynx, and hypodermal and neuronal cell nuclei.
Soft X-ray contact microscopi of nematode Caenorhabditis elegans / G. Poletti, F. Orsini, D. Batani, A. Bernardinello, T. Desai, J. Ullschmied, J. Skala, B. Kralikova, E. Krousky, L. Juha, M. Pfeifer, C. Kadlec, T. Mocek, A. Prag, O. Renner, F. Cotelli, C. Lora Lamia Donin, A. Zullini. - In: THE EUROPEAN PHYSICAL JOURNAL. D, ATOMIC, MOLECULAR AND OPTICAL PHYSICS. - ISSN 1434-6060. - 30:2(2004), pp. 235-241.
Titolo: | Soft X-ray contact microscopi of nematode Caenorhabditis elegans |
Autori: | |
Settore Scientifico Disciplinare: | Settore BIO/05 - Zoologia Settore BIO/06 - Anatomia Comparata e Citologia |
Data di pubblicazione: | 2004 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1140/epjd/e2004-00084-4 |
Appare nelle tipologie: | 01 - Articolo su periodico |