Soft X-ray Contact Microscopy (SXCM) of Caenorhabditis elegans nematodes with typical length ∼800μ m and diameter ∼30μ m has been performed using the PALS laser source of wavelength λ=1.314μ m and pulse duration τ(FWHM)=400 ps. Pulsed soft X-rays were generated using molybdenum and gold targets with laser intensities I≥1014 W/cm2. Images have been recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Cuticle features and several internal organs have been identified in the SXCM images including lateral field, cuticle annuli, pharynx, and hypodermal and neuronal cell nuclei.
Soft X-ray contact microscopy of nematode Caenorhabditis elegans / G. Poletti, F. Orsini, D. Batani, A. Bernardinello, T. Desai, J. Ullschmied, J. Skala, B. Kralikova, E. Krousky, L. Juha, M. Pfeifer, C. Kadlec, T. Mocek, A. Präg, O. Renner, F. Cotelli, C. Lora Lamia, A. Zullini. - In: THE EUROPEAN PHYSICAL JOURNAL. D, ATOMIC, MOLECULAR AND OPTICAL PHYSICS. - ISSN 1434-6060. - 30:2(2004), pp. 235-241. [10.1140/epjd/e2004-00084-4]
Soft X-ray contact microscopy of nematode Caenorhabditis elegans
G. PolettiPrimo
;F. OrsiniSecondo
;F. Cotelli;
2004
Abstract
Soft X-ray Contact Microscopy (SXCM) of Caenorhabditis elegans nematodes with typical length ∼800μ m and diameter ∼30μ m has been performed using the PALS laser source of wavelength λ=1.314μ m and pulse duration τ(FWHM)=400 ps. Pulsed soft X-rays were generated using molybdenum and gold targets with laser intensities I≥1014 W/cm2. Images have been recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Cuticle features and several internal organs have been identified in the SXCM images including lateral field, cuticle annuli, pharynx, and hypodermal and neuronal cell nuclei.File | Dimensione | Formato | |
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