Ethylenethiourea (ETU) is a major metabolite of ethylenebisdithiocarbamate pesticides: a sensitive and specific assay for its determination in human urine is proposed below. ETU is extracted on a diatomaceous earth column using dichloromethane and derivatized with the mixture of N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide and tert-butyldimethyilsilyl chloride. The derivative is analyzed using GC/MS in the EI/SIM mode. The whole procedure is carried out in the presence of ethylenethiourea-d(4) as internal standard. The analytical features of the method are: high specificity, >90% recovery, range of linearity 0-200 microg/L, within- and between-run precision as coefficient of variation, <17 and <20%, respectively, limit of quantification 2 microg/L. In specimens stored in the dark at -20 degrees C ETU is stable for at least 6 months. The procedure was successfully applied to the biological monitoring of vineyard workers exposed to EBDTC and of a matched group of subjects from the general population.

Application of gas chromatography-mass spectrometry for the determination of urinary ethylenethiourea in humans / S. Fustinoni, L. Campo, C. Colosio, S. Birindelli, V. Foà. - In: JOURNAL OF CHROMATOGRAPHY. B. - ISSN 1570-0232. - 814:2(2005 Jan), pp. 251-258. [10.1016/j.jchromb.2004.10.042]

Application of gas chromatography-mass spectrometry for the determination of urinary ethylenethiourea in humans

S. Fustinoni
Primo
;
L. Campo
Secondo
;
C. Colosio;V. Foà
Ultimo
2005

Abstract

Ethylenethiourea (ETU) is a major metabolite of ethylenebisdithiocarbamate pesticides: a sensitive and specific assay for its determination in human urine is proposed below. ETU is extracted on a diatomaceous earth column using dichloromethane and derivatized with the mixture of N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide and tert-butyldimethyilsilyl chloride. The derivative is analyzed using GC/MS in the EI/SIM mode. The whole procedure is carried out in the presence of ethylenethiourea-d(4) as internal standard. The analytical features of the method are: high specificity, >90% recovery, range of linearity 0-200 microg/L, within- and between-run precision as coefficient of variation, <17 and <20%, respectively, limit of quantification 2 microg/L. In specimens stored in the dark at -20 degrees C ETU is stable for at least 6 months. The procedure was successfully applied to the biological monitoring of vineyard workers exposed to EBDTC and of a matched group of subjects from the general population.
Biological monitoring; Occupational exposure; Urinary ethylenethiourea
Settore MED/44 - Medicina del Lavoro
gen-2005
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/14532
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