Background/Aims: Cholangiocarcinoma is a devastating tumour with a poor prognosis. An efficient therapy is unavailable in unoperable patients and new drugs are widely sought for and required. Resveratrol (RES) is a natural molecule with a reported anticancer effect, evaluated on different tumour cell lines. We tested the efficacy of RES on a cholangiocarcinoma cell line for the first time. Methods: We used the human SK-ChA-1 cell line, cultured in the classical two-dimensional model and in the three-dimensional spheroids. After RES exposure morphology, cell viability (colony-forming assay), lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and cancer antigen (CA) 19-9 medium releases, cellular transglutaminase activity, karyotype and cell cycle were evaluated. Results: Resveratrol inhibited cell growth in both the cell culture systems used (from −15 to −80% vs untreated controls) and induced a 40-fold increase of LDH and ALP activities in the culture medium. Also, transglutaminase (TG) activity increased in the cell lysates, together with a cell cycle perturbation characterised by an accumulation in the G1/S phase. Karyotype and CA 19-9 expression were not influenced by the treatment. Conclusions: The observed cytotoxic effect of RES on the human cholangiocarcinoma SK-ChA-1 cell line cultured two- and three-dimensionally suggests to further analyse its chemotherapic/chemopreventive possibilities for this kind of cancer.

Resveratrol inhibits cell growth in a human cholangiocarcinoma cell line / L.R. Roncoroni, L. Elli, E. Dolfini, E. Erba, E. Dogliotti, C. Terrani, L. Doneda, M.G. Grimoldi, M.T. Bardella. - In: LIVER INTERNATIONAL. - ISSN 1478-3223. - 28:10(2008 Nov), pp. 1426-1436.

Resveratrol inhibits cell growth in a human cholangiocarcinoma cell line

L.R. Roncoroni;L. Elli;E. Dolfini;E. Dogliotti;C. Terrani;L. Doneda;M.G. Grimoldi;M.T. Bardella
2008-11

Abstract

Background/Aims: Cholangiocarcinoma is a devastating tumour with a poor prognosis. An efficient therapy is unavailable in unoperable patients and new drugs are widely sought for and required. Resveratrol (RES) is a natural molecule with a reported anticancer effect, evaluated on different tumour cell lines. We tested the efficacy of RES on a cholangiocarcinoma cell line for the first time. Methods: We used the human SK-ChA-1 cell line, cultured in the classical two-dimensional model and in the three-dimensional spheroids. After RES exposure morphology, cell viability (colony-forming assay), lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and cancer antigen (CA) 19-9 medium releases, cellular transglutaminase activity, karyotype and cell cycle were evaluated. Results: Resveratrol inhibited cell growth in both the cell culture systems used (from −15 to −80% vs untreated controls) and induced a 40-fold increase of LDH and ALP activities in the culture medium. Also, transglutaminase (TG) activity increased in the cell lysates, together with a cell cycle perturbation characterised by an accumulation in the G1/S phase. Karyotype and CA 19-9 expression were not influenced by the treatment. Conclusions: The observed cytotoxic effect of RES on the human cholangiocarcinoma SK-ChA-1 cell line cultured two- and three-dimensionally suggests to further analyse its chemotherapic/chemopreventive possibilities for this kind of cancer.
Chemotherapy; Cholangiocarcinoma; Cholangiocytes; Multicellular tumour spheroids; Resveratrol
Settore MED/08 - Anatomia Patologica
Settore BIO/13 - Biologia Applicata
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/142374
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