A method suitable for the determination of 3-nitrotyrosine, a well known marker of nitro-oxidative stress, in plasma by GC-ECD has been investigated. After deproteinisation, SPE and LC separation, 3-nitrotyrosine was derivatised by a single-step procedure to the corresponding N-heptafluorobutyryl heptafluorobutyl ester and analysed. The precision and reliability of the procedure were assured by using 4-nitrophenylalanine as internal standard and a diverter valve to protect the detector. The method was applied to the analysis of volunteers' plasma samples.
New Procedure for the Determination of 3-Nitrotyrosine in Plasma by GC–ECD / R. Pavlovic, E. Santaniello, L.M. Chiesa, P.A. Biondi. - In: CHROMATOGRAPHIA. - ISSN 0009-5893. - 70:3-4(2009 Aug), pp. 637-641.
New Procedure for the Determination of 3-Nitrotyrosine in Plasma by GC–ECD
E. SantanielloSecondo
;L.M. ChiesaPenultimo
;P.A. BiondiUltimo
2009
Abstract
A method suitable for the determination of 3-nitrotyrosine, a well known marker of nitro-oxidative stress, in plasma by GC-ECD has been investigated. After deproteinisation, SPE and LC separation, 3-nitrotyrosine was derivatised by a single-step procedure to the corresponding N-heptafluorobutyryl heptafluorobutyl ester and analysed. The precision and reliability of the procedure were assured by using 4-nitrophenylalanine as internal standard and a diverter valve to protect the detector. The method was applied to the analysis of volunteers' plasma samples.
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