Occurrence of green mold disease on Dictyophora rubrovolvata caused by Trichoderma koningiopsis

Dictyophora rubrovolvata is an important edible mushroom that is widely cultivated in China. In 2019, a serious rot disease on D. rubrovolvata was observed in a mushroom production facility located in Ce Heng County, Southwest of Guizhou Province, China. The causal agent was identified as Trichoderma koningiopsis by amplification and sequencing of the internal transcribed spacer (ITS) region, the translation elongation factor 1-alpha (EF-1 alpha) gene, and the RNA polymerase II subunit (RPB2) gene followed by phylogenetic analysis. Koch's postulates were confirmed by a pathogenicity test that was conducted with healthy D. rubrovolvata, including re-isolation and identification. To our knowledge, this is worldwide the first report of T. koningiopsis as a pathogen on D. rubrovolvata causing green mold disease.