The current coronavirus disease-19 (COVID-19) pandemic, caused by "severe acute respiratory syndrome coronavirus 2" (SARS-CoV-2), underscores the threat posed by newly emerging viruses. The understanding of the mechanisms driving early infection events, that are crucial for the exponential spread of the disease, is mandatory and can be significantly implemented generating 3D in vitro models as experimental platforms to investigate the infection substrates and how the virus invades and ravages the tissues.We here describe a protocol for the creation of a synthetic hydrogel-based 3D culture system that mimics in vitro the complex architectures and mechanical cues distinctive of the upper airway epithelia. We then expose the in vitro generated 3D nasal and tracheal epithelia to gold nanoparticles (AuNPs) that display the typical shape and size distinctive of SARS-CoV-2 and of the majority of Coronaviridae presently known.The infection platform here described provides an efficient and highly physiological in vitro model that reproduces the host-pathogen early interactions, using virus-mimicking nanoparticles, and offers a flexible tool to study virus entry into the cell. At the same time, it reduces the risk of accidental infection/spillovers for researchers, which represents a crucial aspect when dealing with a virus that is highly contagious, virulent, and even deadly.
Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models / G. Pennarossa, A. Fazeli, S. Ledda, F. Gandolfi, T.A. Brevini (METHODS IN MOLECULAR BIOLOGY). - In: Next Generation Culture Platforms for Reliable In Vitro Models / [a cura di] T.A.L. Brevini, A. Fazeli, K. Turksen. - [s.l] : Humana, 2021. - ISBN 9781071612453. - pp. 131-138
Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models
G. PennarossaPrimo
;F. Gandolfi;T.A. Brevini
Ultimo
2021
Abstract
The current coronavirus disease-19 (COVID-19) pandemic, caused by "severe acute respiratory syndrome coronavirus 2" (SARS-CoV-2), underscores the threat posed by newly emerging viruses. The understanding of the mechanisms driving early infection events, that are crucial for the exponential spread of the disease, is mandatory and can be significantly implemented generating 3D in vitro models as experimental platforms to investigate the infection substrates and how the virus invades and ravages the tissues.We here describe a protocol for the creation of a synthetic hydrogel-based 3D culture system that mimics in vitro the complex architectures and mechanical cues distinctive of the upper airway epithelia. We then expose the in vitro generated 3D nasal and tracheal epithelia to gold nanoparticles (AuNPs) that display the typical shape and size distinctive of SARS-CoV-2 and of the majority of Coronaviridae presently known.The infection platform here described provides an efficient and highly physiological in vitro model that reproduces the host-pathogen early interactions, using virus-mimicking nanoparticles, and offers a flexible tool to study virus entry into the cell. At the same time, it reduces the risk of accidental infection/spillovers for researchers, which represents a crucial aspect when dealing with a virus that is highly contagious, virulent, and even deadly.File | Dimensione | Formato | |
---|---|---|---|
Pennarossa et al_MIMB Covid.pdf
accesso aperto
Tipologia:
Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione
171.13 kB
Formato
Adobe PDF
|
171.13 kB | Adobe PDF | Visualizza/Apri |
Pennarossa2021_Protocol_UseOfVirus-MimickingNanopartic.pdf
accesso riservato
Tipologia:
Publisher's version/PDF
Dimensione
277.89 kB
Formato
Adobe PDF
|
277.89 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.