SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments and vaccines. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokines production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune booster of the antiviral immune response with moderate effects against SARS-CoV-2 infection.
Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro / C. Salaris, M. Scarpa, M. Elli, A. Bertolini, S. Guglielmetti, F. Pregliasco, C. Blandizzi, P. Brun, I. Castagliuolo. - In: NUTRIENTS. - ISSN 2072-6643. - 13:2(2021 Feb), pp. 328.1-328.12.
Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro
S. Guglielmetti;F. Pregliasco;
2021
Abstract
SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments and vaccines. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokines production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune booster of the antiviral immune response with moderate effects against SARS-CoV-2 infection.File | Dimensione | Formato | |
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