We present our results in the identification of polymorphic sites within the second exon of the human leukocyte antigen A (HLA-A) region using the DNA microarray technology. Allele specific detection was performed by polymerase chain reaction followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. By this approach we confirmed 32 human samples previously characterized by direct DNA sequencing, thus demonstrating the interest of this approach.
Detection of HLA polymorphisms by ligase detection reaction and a universal array format: a pilot study for low resolution genotyping / C. Consolandi, E. Busti, C. Pera, L. Delfino, G.B. Ferrara, R. Bordoni, B. Castiglioni, L. Rossi Bernardi, C. Battaglia, G. De Bellis. - In: HUMAN IMMUNOLOGY. - ISSN 0198-8859. - 64:1(2003 Jan), pp. 168-178.
Detection of HLA polymorphisms by ligase detection reaction and a universal array format: a pilot study for low resolution genotyping
C. ConsolandiPrimo
;R. Bordoni;L. Rossi Bernardi;C. BattagliaPenultimo
;
2003
Abstract
We present our results in the identification of polymorphic sites within the second exon of the human leukocyte antigen A (HLA-A) region using the DNA microarray technology. Allele specific detection was performed by polymerase chain reaction followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. By this approach we confirmed 32 human samples previously characterized by direct DNA sequencing, thus demonstrating the interest of this approach.
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