We have measured protein kinase C (PKC) activity in cytosolic fractions of leech central nervous system. Phosphatidylserine and oleylacetylglycerol enhanced the activity of the enzyme in a dose-dependent manner even in the absence of calcium. Most of the substrates of the calcium-dependent protein kinase are substrates of the PKC. H-7 and polymyxin B inhibited both PKC and Ca 2+-dependent protein kinase.
Protein kinase C activity in the central nervous system of Hirudo medicinalis / M. Garcia-Gil, G. Cipollini, M. Cattani, D. Bottai, M. Brunelli. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. B, COMPARATIVE BIOCHEMISTRY. - ISSN 0305-0491. - 99:1(1991), pp. 65-70. [10.1016/0305-0491(91)90008-2]
Protein kinase C activity in the central nervous system of Hirudo medicinalis
D. BottaiPenultimo
;
1991
Abstract
We have measured protein kinase C (PKC) activity in cytosolic fractions of leech central nervous system. Phosphatidylserine and oleylacetylglycerol enhanced the activity of the enzyme in a dose-dependent manner even in the absence of calcium. Most of the substrates of the calcium-dependent protein kinase are substrates of the PKC. H-7 and polymyxin B inhibited both PKC and Ca 2+-dependent protein kinase.
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