KNOX genes play an important role in meristematic activity and correct spatial-temporal regulation is crucial for the development of lateral organs. In barley, duplication of a 305 bp element in intron IV of the Barley knox 3 gene (Bkn3) causes the dominant Hooded (K) phenotype: ectopic expression of Bkn3 in the lemma-awn transition zone leads to the formation of a new meristem that develops into an epiphyillic flower. A screen aimed at isolating putative regulators of the BKn3 gene uncovered 4 proteins capable of interacting with the 305 bp element both in yeast and in vitro - K Intron Binding Proteins (KIBPs). Based on their homology with regulatory proteins involved in ethylene response, we investigated the interactions between two of the KIBP genes, ethylene and Bkn3. Ethylene can partially suppress the K phenotype, upregulate KIBP and downregulate Bkn3 gene expression. Overexpression of barley KIBP genes in rice results in altered expression of an ethylene biosynthesis gene and developmental defects consistent with perturbations in ethylene levels. Reporter-effectors assays in protoplasts indicate that different KIBPs may play contrasting roles in intron-mediated regulation. Together, these results suggest ethylene modulates Bkn3 expression via a complex mechanism involving intron-located sequences and repression/activation by different KIBPs.

Cross Talk Between The KNOX And Ethylene Pathways Is Mediated By Intron-Binding Transcription Factors In Barley / M. Osnato, S. Curiale, D. Meynard, E. Guiderdoni, F. Salamini, L. Rossini - In: Plant & Animal Genomes 17. Conference[s.l] : null, 2009 Jan 10. - pp. P738 (( Intervento presentato al 17. convegno Plant & Animal Genomes Conference tenutosi a San Diego nel 2009.

Cross Talk Between The KNOX And Ethylene Pathways Is Mediated By Intron-Binding Transcription Factors In Barley

M. Osnato
Primo
;
S. Curiale
Secondo
;
F. Salamini
Penultimo
;
L. Rossini
Ultimo
2009

Abstract

KNOX genes play an important role in meristematic activity and correct spatial-temporal regulation is crucial for the development of lateral organs. In barley, duplication of a 305 bp element in intron IV of the Barley knox 3 gene (Bkn3) causes the dominant Hooded (K) phenotype: ectopic expression of Bkn3 in the lemma-awn transition zone leads to the formation of a new meristem that develops into an epiphyillic flower. A screen aimed at isolating putative regulators of the BKn3 gene uncovered 4 proteins capable of interacting with the 305 bp element both in yeast and in vitro - K Intron Binding Proteins (KIBPs). Based on their homology with regulatory proteins involved in ethylene response, we investigated the interactions between two of the KIBP genes, ethylene and Bkn3. Ethylene can partially suppress the K phenotype, upregulate KIBP and downregulate Bkn3 gene expression. Overexpression of barley KIBP genes in rice results in altered expression of an ethylene biosynthesis gene and developmental defects consistent with perturbations in ethylene levels. Reporter-effectors assays in protoplasts indicate that different KIBPs may play contrasting roles in intron-mediated regulation. Together, these results suggest ethylene modulates Bkn3 expression via a complex mechanism involving intron-located sequences and repression/activation by different KIBPs.
10-gen-2009
http://www.intl-pag.org/17/abstracts/P07c_PAGXVII_738.html
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/65722
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