Supplementation of leptin in maturation medium and effects on maturation, fertilization and development of horse oocytes after ICSI : immunocytochemical aspects

A critical analysis of the results of research in the function of the endometrium was carried out and a view point presented. The role of the endometrium in endocrine regulation of the oestrus cycle can be summarized as follows: 1. The transfer of prostaglandin F2α (PGF 2α) from the uterus to an ovary, which causes luteolysis, occurs mainly via the lymphatic pathways. 2. The system of retrograde transfer of PGs enables PGF2α and PGE2 to reach the myometrium and endometrium with arterial blood at high concentration. In the luteal phase, PGF2α, together with the increasing concentration of progesterone, constricts the arterial vessels of the uterus; in the follicular phase and in early pregnancy, PGE2 together with oestrogen and embryonic signals, relaxes the arterial vessels. In addition, this system protects the corpus luteum from premature luteolysis during the cycle and luteolysis during early pregnancy. 3. In days 10-12 of the cycle, the blood flow in the uterus decreases by 60-70% in pigs and around 90% in sheep. This causes ischaemia and local hypoxia confirmed by the presence of hypoxia inducible factor and thus remodelling of the endometrium commences. 4. The pulsatile elevations in PGF2α concentration occurring in the blood flowing out of the uterus during the period of luteolysis and the next few days, do not result from increased PGF2α synthesis as suggested in numerous studies. They are the effect of excretion of PGF2α and its metabolites together with lymph and venous blood and tissue fluids in which prostaglandin accumulates.