The biosynthesis and release of neuropeptide Y (NPY) is regulated by several factors. Here, the effect of the muscarinic agonist carbachol on NPY biosynthesis and release was analyzed utilizing the SH-SY5Y human neuroblastoma cell line. We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). In conclusion, the present observations support the hypothesis that muscarinic receptor activation regulates the biosynthesis and secretion of NPY.
Cholinergic regulation of neuropeptide Y synthesis and release in human neuroblastorna cells / E. Dozio, M. Ruscica, D. Feltrin, M. Motta, P. Magni. - In: PEPTIDES. - ISSN 0196-9781. - 29:3(2008 Mar), pp. 491-495.
Cholinergic regulation of neuropeptide Y synthesis and release in human neuroblastorna cells
E. DozioPrimo
;M. RuscicaSecondo
;M. MottaPenultimo
;P. MagniUltimo
2008
Abstract
The biosynthesis and release of neuropeptide Y (NPY) is regulated by several factors. Here, the effect of the muscarinic agonist carbachol on NPY biosynthesis and release was analyzed utilizing the SH-SY5Y human neuroblastoma cell line. We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). In conclusion, the present observations support the hypothesis that muscarinic receptor activation regulates the biosynthesis and secretion of NPY.File | Dimensione | Formato | |
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