Evaluation of Pseudomonas cepacia lipase (PCL) activity by a titrimetric method with triacylglycerols (TAG) and synthetic dialkylglycerol esters (DAGE) established the chain length selectivity of the enzyme and this information has been used to design a new chromogenic substrate [1,2-di-O-octyl-sn-glycerol-3-O-(4-nitrophenyl) glutarate] for the determination of the lipolytic activity of PCL.

Spectrophotometric assay of lipase activity: A new 4-nitrophenyl ester of a dialkylglycerol suitable as a chromogenic substrate of Pseudomonas cepacia lipase. / P. Ciuffreda, S. Casati, A. Loseto, E. Santaniello. - In: BIOCATALYSIS AND BIOTRANSFORMATION. - ISSN 1024-2422. - 21:3(2003), pp. 123-127.

Spectrophotometric assay of lipase activity: A new 4-nitrophenyl ester of a dialkylglycerol suitable as a chromogenic substrate of Pseudomonas cepacia lipase.

P. Ciuffreda
Primo
;
S. Casati
Secondo
;
E. Santaniello
Ultimo
2003

Abstract

Evaluation of Pseudomonas cepacia lipase (PCL) activity by a titrimetric method with triacylglycerols (TAG) and synthetic dialkylglycerol esters (DAGE) established the chain length selectivity of the enzyme and this information has been used to design a new chromogenic substrate [1,2-di-O-octyl-sn-glycerol-3-O-(4-nitrophenyl) glutarate] for the determination of the lipolytic activity of PCL.
4-Nitrophenyl esters; Dialkylglycerol esters; Lipase activity; Pseudomonas cepacia lipase; Spectrophotometric assay
Settore BIO/10 - Biochimica
2003
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/5139
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact