High-resolution X-ray microtomography for three-dimensional visualization of human stem cell muscle homing

In the perspective of clin. translation of stem cell research, it would be advantageous to develop new techniques to detect donor cells after transplantation to track their fate and thus better understand their role in regeneration of damaged and diseased tissues. In this study we use X-ray computed microtomog. for three-dimensional visualization of stem cells that were labeled with magnetic nanoparticles and transplanted via intra-arterial infusion. We show that X-ray computed microtomog. offers the possibility to detect with high definition and resoln. human cells after transplantation, and opens new possibilities for both exptl. stem cell research.