We investigated the presence of lucocorticoid receptors (GR) as well as the role of glucocorticoids (Gc) in the control of proliferation of the androgen-independent prostate cancer cell line, DU145. We detected the presence of a specific high affinity binding site (Kd 2·3 nM) for [3H]dexamethasone ([3H]Dex) in the cytosolic preparations of DU145 cells; the density of these binding sites is significantly higher than that detected in HA22T/ VGH and in HepG2, two hepatoma cell lines classically considered models for the study of GR. Immunocytochemistry studies confirmed the presence of GR in the cytosolic compartment of DU145 cells; GR undergo translocation to the nucleus following exposure to dexamethasone (Dex). The functional activity of GR present in DU145 cells was also studied by analyzing the potency of Dex in inducing chloramphenicol acyltransferase (CAT) activity in DU145 cells transfected with a glucocorticoid/progesterone response element (GRE/PRE) tkCAT plasmid (GRE/PREtkCAT plasmid). The results have shown that Dex stimulates the transcriptional activity of GR in transfected DU145 cells with an EC50 of 9·65 nM and a maximal induction of sevenfold above basal levels. Finally, a dose-dependent (IC50 3·14 nM) decrease of DU145 cell numbers was observed after their exposure to Dex for 4 days; this effect was counteracted by the presence of the steroid antagonist, RU486. In conclusion, the present data suggest a possible role of corticoids in the control of the growth of androgen-independent prostate cancer

Expression and role of functional glucocorticoid receptors in the human androgen-independent prostate cancer cell line, DU145 / D. Dondi, R. Maggi, E. Scaccianoce, L. Martini, M. Motta, A. Poletti. - In: JOURNAL OF MOLECULAR ENDOCRINOLOGY. - ISSN 0952-5041. - 26:3(2001 Jan 08), pp. 185-191. [10.1677/jme.0.0260185]

Expression and role of functional glucocorticoid receptors in the human androgen-independent prostate cancer cell line, DU145

D. Dondi
Primo
;
R. Maggi
Secondo
;
L. Martini;M. Motta
Penultimo
;
A. Poletti
Ultimo
2001

Abstract

We investigated the presence of lucocorticoid receptors (GR) as well as the role of glucocorticoids (Gc) in the control of proliferation of the androgen-independent prostate cancer cell line, DU145. We detected the presence of a specific high affinity binding site (Kd 2·3 nM) for [3H]dexamethasone ([3H]Dex) in the cytosolic preparations of DU145 cells; the density of these binding sites is significantly higher than that detected in HA22T/ VGH and in HepG2, two hepatoma cell lines classically considered models for the study of GR. Immunocytochemistry studies confirmed the presence of GR in the cytosolic compartment of DU145 cells; GR undergo translocation to the nucleus following exposure to dexamethasone (Dex). The functional activity of GR present in DU145 cells was also studied by analyzing the potency of Dex in inducing chloramphenicol acyltransferase (CAT) activity in DU145 cells transfected with a glucocorticoid/progesterone response element (GRE/PRE) tkCAT plasmid (GRE/PREtkCAT plasmid). The results have shown that Dex stimulates the transcriptional activity of GR in transfected DU145 cells with an EC50 of 9·65 nM and a maximal induction of sevenfold above basal levels. Finally, a dose-dependent (IC50 3·14 nM) decrease of DU145 cell numbers was observed after their exposure to Dex for 4 days; this effect was counteracted by the presence of the steroid antagonist, RU486. In conclusion, the present data suggest a possible role of corticoids in the control of the growth of androgen-independent prostate cancer
Settore BIO/09 - Fisiologia
Settore MED/13 - Endocrinologia
Settore BIO/13 - Biologia Applicata
8-gen-2001
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/28954
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 22
  • ???jsp.display-item.citation.isi??? 20
social impact