The embryotoxic potential of chlorpyrifos (CPF) and malathion (MTN), two organ ophosphorus insecticides (OPs), was evaluated by modified Frog Embryo Teratogenesis Assay-Xenopus (FETAX). CPF and MTN were not embryolethal even at the highest concentration tested (6000 mug/l), but both exhibited a powerful teratogenicity. The probit analysis of malformed larva percentages showed a TC50 of 161.54 mug/l for CPF, and a TC50 of 2394.01 mug/l for MTN. Therefore, CPF teratogenicity was about 15 times higher than MTN. Larvae of both exposed groups were mainly affected by ventral and/or lateral tail flexure coupled with abnormal gut coiling. Histopathological diagnosis displayed abnormal myotomes and myocytes with marked hypertrophics localized at the cell extremity, probably due to a break away of myofibril extremities at the intersomitic junction level. We speculate that this muscular damage was related to inhibition of acetylcholinesterase that showed a clear concentration-response in CPF and MTN exposed larvae. The teratogenic effects of these anti-cholinesterase compounds on Xenopus laevis myogenesis suggest a possible role played by OPs on induction of congenital muscular dystrophy.

Comparative teratogenicity of Chlorpyrifos and Malathion on Xenopus laevis development / P. Bonfanti, A. Colombo, F. Orsi, I. Nizzetto, M. Andrioletti, R. Bacchetta, P. Mantecca, U. Fascio, G. Vailati, C. Vismara. - In: AQUATIC TOXICOLOGY. - ISSN 0166-445X. - 70:3(2004), pp. 189-200.

Comparative teratogenicity of Chlorpyrifos and Malathion on Xenopus laevis development

M. Andrioletti;R. Bacchetta;P. Mantecca;U. Fascio;G. Vailati
Penultimo
;
C. Vismara
Ultimo
2004

Abstract

The embryotoxic potential of chlorpyrifos (CPF) and malathion (MTN), two organ ophosphorus insecticides (OPs), was evaluated by modified Frog Embryo Teratogenesis Assay-Xenopus (FETAX). CPF and MTN were not embryolethal even at the highest concentration tested (6000 mug/l), but both exhibited a powerful teratogenicity. The probit analysis of malformed larva percentages showed a TC50 of 161.54 mug/l for CPF, and a TC50 of 2394.01 mug/l for MTN. Therefore, CPF teratogenicity was about 15 times higher than MTN. Larvae of both exposed groups were mainly affected by ventral and/or lateral tail flexure coupled with abnormal gut coiling. Histopathological diagnosis displayed abnormal myotomes and myocytes with marked hypertrophics localized at the cell extremity, probably due to a break away of myofibril extremities at the intersomitic junction level. We speculate that this muscular damage was related to inhibition of acetylcholinesterase that showed a clear concentration-response in CPF and MTN exposed larvae. The teratogenic effects of these anti-cholinesterase compounds on Xenopus laevis myogenesis suggest a possible role played by OPs on induction of congenital muscular dystrophy.
chlorpyrifos ; FETAX ; malathion ; muscular damage
Settore BIO/06 - Anatomia Comparata e Citologia
2004
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/26339
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