The plasma membrane is a highly dynamic cell-barrier if the nature and distribution of its constituents are considered. Ion channels are embedded in these double lipid bilayers, which modulate their 3D-structures. The structure modulations by the lipid bilayer can assume such a degree that channel activation depends on them, as was shown for the KcsA potassium channel. Here we show that the cation-over-anion selectivity of reconstituted ICln channels can be varied by the thickness of a bilayer build of phosphatidylcholines. The shorter the acyl-chains and therefore the thinner the bilayers of the membrane are, the more potassium selective the channels are. In contrast, the longer the acyl-chains and therefore the thicker the membranes are, the more chloride selective the channels become.

Membrane thickness changes ion-selectivity of channel-proteins / M. Garavaglia, S. Dopinto, M. Ritter, J. Furst, S. Saino, F. Guizzardi, M. Jakab, C. Bazzini, V. Vezzoli, S. Dossena, S. Rodighiero, C. Sironi, G. Bottà, G. Meyer, R. Henderson, M. Paulmichl. - In: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY. - ISSN 1015-8987. - 14:4-6(2004), pp. 231-240.

Membrane thickness changes ion-selectivity of channel-proteins

M. Garavaglia
Primo
;
S. Dopinto
Secondo
;
S. Saino;F. Guizzardi;C. Bazzini;V. Vezzoli;S. Dossena;S. Rodighiero;C. Sironi;G. Bottà;G. Meyer;M. Paulmichl
Ultimo
2004

Abstract

The plasma membrane is a highly dynamic cell-barrier if the nature and distribution of its constituents are considered. Ion channels are embedded in these double lipid bilayers, which modulate their 3D-structures. The structure modulations by the lipid bilayer can assume such a degree that channel activation depends on them, as was shown for the KcsA potassium channel. Here we show that the cation-over-anion selectivity of reconstituted ICln channels can be varied by the thickness of a bilayer build of phosphatidylcholines. The shorter the acyl-chains and therefore the thinner the bilayers of the membrane are, the more potassium selective the channels are. In contrast, the longer the acyl-chains and therefore the thicker the membranes are, the more chloride selective the channels become.
Bilayer; DAPC; Diph-PC; DLPC; DMPC; DPPC; DSPC; ICln; Reconstitution; Volume regulation
Settore BIO/09 - Fisiologia
2004
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/26283
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