In this study, cytogenetic analysis of an infertile mare revealed a 64, XY karyotype. The XY sex-reversed animal had a female phenotype with gonadal dysgenesis. Using Southern blot analysis, we tested for the presence of two Y-specific genes SRY and ZFY by using DNA isolated from peripheral blood leukocytes. The results showed that at least the DNA-binding domain of the SRY gene was deleted from the Y chromosome of the XY mare but that the ZFY gene was present on this chromosome.
Molecular analysis of a XY mare with gonadal dysgenesis / E. Pailhoux, EP. Cribiu, P. Parma, C. Cotinot. - In: HEREDITAS. - ISSN 0018-0661. - 122:2(1995), pp. 109-112. [10.1111/j.1601-5223.1995.00109.x]
Molecular analysis of a XY mare with gonadal dysgenesis
P. ParmaPenultimo
;
1995
Abstract
In this study, cytogenetic analysis of an infertile mare revealed a 64, XY karyotype. The XY sex-reversed animal had a female phenotype with gonadal dysgenesis. Using Southern blot analysis, we tested for the presence of two Y-specific genes SRY and ZFY by using DNA isolated from peripheral blood leukocytes. The results showed that at least the DNA-binding domain of the SRY gene was deleted from the Y chromosome of the XY mare but that the ZFY gene was present on this chromosome.
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