It is well known that water disinfection through chlorination causes the formation of a mixt. of disinfection byproducts (DBPs), many of which are genotoxic and carcinogenic. To demonstrate the formation of such compds., a pilot water plant supplied with water from Lake Trasimeno was set up at the waterworks of Castiglione del Lago (PG, Italy). The disinfectants, continuously added to prefiltered lake water flowing into three different basins, were sodium hypochlorite, chlorine dioxide and peracetic acid, an alternative disinfectant used until now for disinfecting waste waters, but not yet studied for a possible use in drinking water treatment. The aim of this study was to evaluate the formation during the disinfection processes of some toxic compds. that could explain the genotoxic effects of drinking waters. Differently treated waters were concd. by solid-phase adsorption on silica C18 columns and toxicity was assessed in a line of human hepatoma cells (HepG2), a metabolically competent cellular line very useful for human risk evaluation. The seasonal variability of the phys.-chem. water characteristics (AOX, UV 254 nm, potential formation of THM, pH and temp.) made indispensable experimentation with water samples taken during the various seasons. Autumn waters cause greater toxicity compared to those of other seasons, in particular diln. of the conc. at 0.5 l equiv. of disinfected waters with chlorine dioxide and peracetic acid causes a 55% redn. in cellular vitality while the cellular vitality is over 80% with the all other water concs. Moreover it is very interesting underline that non-cytotoxic quantities of the autumnal water concs. cause, after 2 h treatment, a decrease in GSH and a statistically significant increase in oxygen radicals, while after prolonged treatment (24 h) cause a GSH increase, without variations in the oxygen radical content. This phenomenon could be interpreted as the cellular adaptation response to an initial oxidative stress. [on SciFinder (R)]

Toxicity evaluation of surface water treated with different disinfectants in HepG2 cells / L. Marabini, S. Frigerio, E. Chiesara, S. Radice. - In: WATER RESEARCH. - ISSN 0043-1354. - 40:2(2006 Jan), pp. 267-272.

Toxicity evaluation of surface water treated with different disinfectants in HepG2 cells

L. Marabini
Primo
;
S. Frigerio
Secondo
;
E. Chiesara
Penultimo
;
S. Radice
Ultimo
2006

Abstract

It is well known that water disinfection through chlorination causes the formation of a mixt. of disinfection byproducts (DBPs), many of which are genotoxic and carcinogenic. To demonstrate the formation of such compds., a pilot water plant supplied with water from Lake Trasimeno was set up at the waterworks of Castiglione del Lago (PG, Italy). The disinfectants, continuously added to prefiltered lake water flowing into three different basins, were sodium hypochlorite, chlorine dioxide and peracetic acid, an alternative disinfectant used until now for disinfecting waste waters, but not yet studied for a possible use in drinking water treatment. The aim of this study was to evaluate the formation during the disinfection processes of some toxic compds. that could explain the genotoxic effects of drinking waters. Differently treated waters were concd. by solid-phase adsorption on silica C18 columns and toxicity was assessed in a line of human hepatoma cells (HepG2), a metabolically competent cellular line very useful for human risk evaluation. The seasonal variability of the phys.-chem. water characteristics (AOX, UV 254 nm, potential formation of THM, pH and temp.) made indispensable experimentation with water samples taken during the various seasons. Autumn waters cause greater toxicity compared to those of other seasons, in particular diln. of the conc. at 0.5 l equiv. of disinfected waters with chlorine dioxide and peracetic acid causes a 55% redn. in cellular vitality while the cellular vitality is over 80% with the all other water concs. Moreover it is very interesting underline that non-cytotoxic quantities of the autumnal water concs. cause, after 2 h treatment, a decrease in GSH and a statistically significant increase in oxygen radicals, while after prolonged treatment (24 h) cause a GSH increase, without variations in the oxygen radical content. This phenomenon could be interpreted as the cellular adaptation response to an initial oxidative stress. [on SciFinder (R)]
Chlorine dioxide; Disinfection by-products (DBPs); HepG2 cells; Oxidative stress; Peracetic acid; Sodium hypochlorite; Water disinfection
Settore BIO/14 - Farmacologia
gen-2006
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/22392
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