We analyzed the role of IL-6 in the protection that ischemic preconditioning (IP) exerts against hepatic ischemia reperfusion-mediated (I/R) oxidative damage, particularly in fatty livers. IP-related IL-6 up-regulation during reperfusion in steatotic and non-steatotic livers was correlated with reduced indexes of liver damage, as also demonstrated by pharmacol. modulation of IL-6. IP activated NF-kB and HSF during ischemia (Isc), whereas AP-1 activity was unaffected. IP blunted the activation of STAT3 and stress-responsive genes, such as NF-kB, AP-1 and heme oxygenase (HO-1) during reperfusion. The role of reduced oxidative stress in hepatoprotection of fatty livers was further demonstrated by the fact that: (i) IP prevented the decrease of glutathione levels and the increase of lipid peroxidn.; (ii) the anti-oxidant GSH-ester prevented lipid peroxidn. and necrosis. In conclusion, IP modulates the activity of transcription factors and triggers IL-6 prodn.; this may prevent hepatic I/R damage in a oxidative stress-dependent way, particularly in fatty livers.

Up regulation of IL-6 by ischemic preconditioning in normal and fatty rat livers: association with reduction of oxidative stress / L. Tacchini, G. Cairo, C. De Ponti, M. Massip, J. Rosello-Catafau, C. Peralta. - In: FREE RADICAL RESEARCH. - ISSN 1071-5762. - 40:11(2006), pp. 1206-1217. [10.1080/10715760600885432]

Up regulation of IL-6 by ischemic preconditioning in normal and fatty rat livers: association with reduction of oxidative stress

L. Tacchini
Primo
;
G. Cairo
Secondo
;
2006

Abstract

We analyzed the role of IL-6 in the protection that ischemic preconditioning (IP) exerts against hepatic ischemia reperfusion-mediated (I/R) oxidative damage, particularly in fatty livers. IP-related IL-6 up-regulation during reperfusion in steatotic and non-steatotic livers was correlated with reduced indexes of liver damage, as also demonstrated by pharmacol. modulation of IL-6. IP activated NF-kB and HSF during ischemia (Isc), whereas AP-1 activity was unaffected. IP blunted the activation of STAT3 and stress-responsive genes, such as NF-kB, AP-1 and heme oxygenase (HO-1) during reperfusion. The role of reduced oxidative stress in hepatoprotection of fatty livers was further demonstrated by the fact that: (i) IP prevented the decrease of glutathione levels and the increase of lipid peroxidn.; (ii) the anti-oxidant GSH-ester prevented lipid peroxidn. and necrosis. In conclusion, IP modulates the activity of transcription factors and triggers IL-6 prodn.; this may prevent hepatic I/R damage in a oxidative stress-dependent way, particularly in fatty livers.
Oxidative stress; Redox reactions; Reperfusion; Transcription factors
Settore MED/04 - Patologia Generale
2006
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/22325
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 22
  • ???jsp.display-item.citation.isi??? 23
social impact