The lipolytic activity of lipases from porcine pancreas (PPL) and Pseudomonas cepacia (PCL) on synthetic dialkylglycerol fatty acyl esters was evaluated by titrimetric method and the observed chain length selectivity constituted the basis for the design of new chromogenic substrates [1,2-di-O-butyl-sn-glycerol-3-(4-nitrophenyl)carbonate and 1,2-di-O-octyl-sn-glycerol-3-(4-nitrophenyl)glutarate] that were shown to be specific for spectrophotometric assay of PPL and PCL activity, respectively. (copyright) 2006 Elsevier B.V. All rights reserved.
Design of new chromogenic substrates for the spectrophotometric assay of lipolytic activity of lipases / E. Santaniello, P. Ciuffreda, S. Casati, H. El Sayed. - In: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC. - ISSN 1381-1177. - 40:3-4(2006), pp. 76-80.
Design of new chromogenic substrates for the spectrophotometric assay of lipolytic activity of lipases
E. SantanielloPrimo
;P. CiuffredaSecondo
;S. CasatiPenultimo
;H. El SayedUltimo
2006
Abstract
The lipolytic activity of lipases from porcine pancreas (PPL) and Pseudomonas cepacia (PCL) on synthetic dialkylglycerol fatty acyl esters was evaluated by titrimetric method and the observed chain length selectivity constituted the basis for the design of new chromogenic substrates [1,2-di-O-butyl-sn-glycerol-3-(4-nitrophenyl)carbonate and 1,2-di-O-octyl-sn-glycerol-3-(4-nitrophenyl)glutarate] that were shown to be specific for spectrophotometric assay of PPL and PCL activity, respectively. (copyright) 2006 Elsevier B.V. All rights reserved.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
