Ratiometric fluorescent sensors were constructed from RNA aptamers by generating modular ribonucleopeptide complexes. Fluorescent ribonucleopeptides contg. fluorophore seminaphthorhodafluor tethered to their peptide subunit revealed a dual emission property, which permitted a ratiometric fluorescent measurement of a substrate-binding event. The strategy successfully afforded ratiometric fluorescent sensors for biol. active small ligands, tetracycline, dopamine and streptomycin.
Construction of ratiometric fluorescent sensors by ribonucleopeptides / C. Annoni, E. Nakata, T. Tamura, F.F. Liew, S. Nakano, M.L. Gelmi, T. Morii. - In: ORGANIC & BIOMOLECULAR CHEMISTRY. - ISSN 1477-0520. - 10:44(2012), pp. 8767-8769.
Construction of ratiometric fluorescent sensors by ribonucleopeptides
C. AnnoniPrimo
;M.L. GelmiPenultimo
;
2012
Abstract
Ratiometric fluorescent sensors were constructed from RNA aptamers by generating modular ribonucleopeptide complexes. Fluorescent ribonucleopeptides contg. fluorophore seminaphthorhodafluor tethered to their peptide subunit revealed a dual emission property, which permitted a ratiometric fluorescent measurement of a substrate-binding event. The strategy successfully afforded ratiometric fluorescent sensors for biol. active small ligands, tetracycline, dopamine and streptomycin.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
