Università degli Studi di Milano   Archivio Istituzionale della Ricerca
Please use this identifier to cite or link to this item: http://hdl.handle.net/2434/214234

Title: Neuritin 1 promotes neuronal migration
Authors: ZITO, ARIANNA (First author)
CARTELLI, DANIELE (Second author)
CAPPELLETTI, GRAZIELLA
CARIBONI, ANNA MARIA
POLETTI, ANGELO (Corresponding)
GALBIATI, MARIARITA (Last author)
Abstract: Neuritin 1 (Nrn1 or cpg15-1) is an activity-dependent protein involved in synaptic plasticity during brain development, a process that relies upon neuronal migration. By analyzing Nrn1 expression, we found that it is highly expressed in a mouse model of migrating immortalized neurons (GN11 cells), but not in a mouse model of non-migrating neurons (GT1-7 cells). We thus hypothesized that Nrn1 might control neuronal migration. By using complementary assays, as Boyden’s microchemotaxis, scratch-wounding and live cell imaging, we found that GN11 cell migration is enhanced when Nrn1 is overexpressed and decreased when Nrn1 is silenced. The effects of Nrn1 in promoting neuronal migration have been then confirmed ex vivo, on rat cortical interneurons, by Boyden chamber assays and focal electroporation of acute embryonic brain slices. Furthermore, we found that Nrn1 level modulation affects GN11 cell morphology. The process is also paralleled by Nrn1-induced a-tubulin post-translational modifications, a well-recognized marker of microtubule stability. Altogether, the data demonstrate a novel function of Nrn1 in promoting migration of neuronal cells and indicate that Nrn1 levels impact on microtubule stability.
Keywords: Neuritin ; Cpg15 ; Cell migration ; GnRH neurons ; Ganglionic eminence ; Microtubule
MIUR subjects: BIO/09 - Fisiologia
BIO/13 - Biologia Applicata
BIO/06 - Anatomia Comparata e Citologia
BIO/16 - Anatomia Umana
Publication date: Jan-2014
Digital Object Identifier (DOI): 10.1007/s00429-012-0487-1
Research centers: Centro Interdipartimentale di Eccellenza per le Malattie Neurodegenerative CEND
Centro Interuniversitario di Ricerca sulle Basi Molecolari delle Malattie Neurodegenerative
Citation: Neuritin 1 promotes neuronal migration / A. Zito, D. Cartelli, G. Cappelletti, A. Cariboni, W. Andrews, J.G. Parnavelas, A. Poletti, M. Galbiati. - In: Brain. - ISSN 1863-2653. - ISSN 1863-2661. - 219:1(2014 Jan), pp. 105-118.
Pubmed identifier: 23212301
Scopus identifier: 84870331007
Type: Article (author)

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