Overexpression of hybrid Virus-Like-Particles (VLP) was found to dramatically affect cell kinetics and morphology with a significant increase in doubling times, induction times and major alterations in cell volume distributions. Optimal expression (ca. 10 mg/l) was obtained with a two-step fermentation strategy (raffinose --> galactose) coupled to GAL4 overexpression, resulting in partial cell lysis and VLP release.

IN SACCHAROMYCES-CEREVISIAE OVEREXPRESSION OF HYBRID VIRUS-LIKE-PARTICLES CORRELATES WITH ALTERED CELL-VOLUME DISTRIBUTIONS / P. CAVADINI, L. POPOLO, M. VAI, R. VARONA, L. GAROFANO, M. VANONI. - In: BIOTECHNOLOGY LETTERS. - ISSN 0141-5492. - 16:11(1994), pp. 1131-1134.

IN SACCHAROMYCES-CEREVISIAE OVEREXPRESSION OF HYBRID VIRUS-LIKE-PARTICLES CORRELATES WITH ALTERED CELL-VOLUME DISTRIBUTIONS

L. POPOLO;
1994

Abstract

Overexpression of hybrid Virus-Like-Particles (VLP) was found to dramatically affect cell kinetics and morphology with a significant increase in doubling times, induction times and major alterations in cell volume distributions. Optimal expression (ca. 10 mg/l) was obtained with a two-step fermentation strategy (raffinose --> galactose) coupled to GAL4 overexpression, resulting in partial cell lysis and VLP release.
Settore BIO/11 - Biologia Molecolare
1994
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/200563
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